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Titolo:
GLYCOPEPTIDE ANTIBIOTIC-RESISTANCE GENES IN GLYCOPEPTIDE-PRODUCING ORGANISMS
Autore:
MARSHALL CG; LESSARD IAD; PARK IS; WRIGHT GD;
Indirizzi:
MCMASTER UNIV,DEPT BIOCHEM,1200 MAIN ST W HAMILTON ON L8N 3Z5 CANADA MCMASTER UNIV,DEPT BIOCHEM HAMILTON ON L8N 3Z5 CANADA HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOL PHARMACOL BOSTON MA 02115
Titolo Testata:
Antimicrobial agents and chemotherapy
fascicolo: 9, volume: 42, anno: 1998,
pagine: 2215 - 2220
SICI:
0066-4804(1998)42:9<2215:GAGIGO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-LACTATE DEHYDROGENASE; ENTEROCOCCUS-FAECIUM BM4147; D-ALANINE LIGASE; DEPSIPEPTIDE PEPTIDOGLYCAN PRECURSORS; 2-COMPONENT REGULATORY SYSTEM; ALANYL-D-ALANINE; VANCOMYCIN-RESISTANCE; LEUCONOSTOC-MESENTEROIDES; BACTERIAL-RESISTANCE; SUBSTRATE-BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
C.G. Marshall et al., "GLYCOPEPTIDE ANTIBIOTIC-RESISTANCE GENES IN GLYCOPEPTIDE-PRODUCING ORGANISMS", Antimicrobial agents and chemotherapy, 42(9), 1998, pp. 2215-2220

Abstract

The mechanism of high-level resistance to vancomycin in enterococci consists of the synthesis of peptidoglycan terminating in D-atanyl-D-lactate instead of the usual D-alanyl-D-alanine. This alternate cell wall biosynthesis pathway is ensured by the collective actions of three enzymes: VanH, VanA, and VanX. The origin of this resistance mechanism is unknown. We have cloned three genes encoding homologs of VanH, VanA, and VanX from two organisms which produce glycopeptide antibiotics: the A47934 producer Streptomyces toyocaensis NRRL 15009 and the vancomycin producer Amycolatopsis orientalis C329.2. The predicted amino acid sequences are highly similar to those found in VRE: 54 to 61% identity for VanH, 59 to 63% identity for VanA, and 61 to 64% identity for VanX. Furthermore, the orientations of the genes, vanH, vanA, and vanX,are identical to the orientations found in vancomycin-resistant enterococci. Southern analysis of total DNA from other glycopeptide-producing organisms, A. orientalis 18098 (chloro-eremomycin producer), A. orientalis subsp. lurida (ristocetin producer), and Amycolatopsis coloradensis subsp. labeda (teicoplanin and avoparcin producer), with a probederived from the vanH, vanA, and vanX fluster from A. orientalis C329.2 revealed cross-hybridizing DNA in all strains. In addition, the vanH, vanA, vanX cluster was amplified from all glycopeptide-producing organisms by PCR with degenerate primers complementary to conserved regions in VanH and VanX. Thus, this gene sequence is common to all glycopeptide producers tested. These results suggest that glycopeptide-producing organisms may have been the source of resistance genes in vancomycin-resistant enterococci.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:17:08