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Titolo:
CYTOSTATIC ACTIVITY DEVELOPS DURING MEIOSIS-I IN OOCYTES OF LT SV MICE/
Autore:
CIEMERYCH MA; KUBIAK JZ;
Indirizzi:
UNIV RENNES 1,BIOL & GENET DEV LAB,CNRS,UPR 41,CAMPUS BEAULIEU F-35042 RENNES FRANCE UNIV WARSAW,INST ZOOL,DEPT EMBRYOL PL-02089 WARSAW POLAND UNIV PARIS 07,CNRS,INST JACQUES MONOD F-75251 PARIS 05 FRANCE
Titolo Testata:
Developmental biology (Print)
fascicolo: 2, volume: 200, anno: 1998,
pagine: 198 - 211
SICI:
0012-1606(1998)200:2<198:CADDMI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURATION-PROMOTING FACTOR; MATURING MOUSE OOCYTES; MEIOTIC CELL-CYCLE; PROTEIN-KINASE-II; MAP KINASE; XENOPUS-OOCYTES; PARTHENOGENETIC DEVELOPMENT; M-PHASE; ACTIVATION; METAPHASE;
Keywords:
CSF; LT/SV MICE; MAP KINASE; MICROTUBULES; MEIOTIC ANAPHASE; MEIOTIC MATURATION; MPF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Ciemerych e J.Z. Kubiak, "CYTOSTATIC ACTIVITY DEVELOPS DURING MEIOSIS-I IN OOCYTES OF LT SV MICE/", Developmental biology (Print), 200(2), 1998, pp. 198-211

Abstract

Oocytes of wild-type mice are ovulated as the secondary oocytes arrested at metaphase of the second meiotic division. Their fertilization or parthenogenetic activation triggers the completion of the second meiotic division followed by the first embryonic interphase. Oocytes of the LT/Sv strain of mice are ovulated either at the first meiotic metaphase (M I) as primary oocytes or in the second meiotic metaphase (M II) as secondary oocytes. We show here that during in vitro maturation ahigh proportion of LT/Sv oocytes progresses normally only until metaphase I. In these oocytes MAP kinase activates shortly after histone H1kinase (MPE) activation and germinal vesicle breakdown. However, MAP kinase activation is slightly earlier than in oocytes from wild-type F1 (CBA/H x C57Bl/10) mice. The first meiotic spindle of these oocytes forms similarly to wild-type oocytes. During aging, however, it increases in size and finally degenerates. In those oocytes which do not remain in metaphase I the extrusion of first polar bodies is highly delayed and starts about 15 h after germinal vesicle breakdown. Most of theoocytes enter interphase directly after first polar body extrusion. Fusion between metaphase I LT/Sv oocytes and wild-type mitotic one-cellembryos results in prolonged M-phase arrest of hybrids in a proportion similar to control LT/Sv oocytes and control hybrids made by fusion of two M I LT/Sv oocytes. This indicates that LT/Sv oocytes develop cytostatic factor during metaphase I. Eventually, anaphase occurs spontaneously and the hybrids extrude the polar body and form pronuclei in aproportion similar as in controls. In hybrids between LT/Sv metaphaseI oocytes and wild-type metaphase II oocytes (which contain cytostatic factor) anaphase I proceeds at the time observed in control LT/Sv oocytes and hybrids between two M I LT/Sv oocytes, and is followed by the parthenogenetic activation and formation of interphase nuclei. Also the great majority of hybrids between M I and M II wild-type oocytes undergoes the anaphase but further arrests in a subsequent M-phase. These observations suggest that an internally triggered anaphase I occursdespite the presence of the cytostatic activity both in LT/Sv and wild-type M I oocytes. Anaphase I triggering mechanism must therefore either inactivate or override the CSF activity. The comparison between spontaneous and induced activation of metaphase I LT/Sv oocytes shows that mechanisms involved in anaphase I triggering are altered in these oocytes. Thus, the prolongation of metaphase I in LT/Sv oocytes seems to be determined by delayed anaphase I triggering and not provoked directly by the cytostatic activity. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 04:14:35