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Titolo:
FLUORESCEIN-LABELED DEXTRAN CONCENTRATION IS INCREASED IN BAL FLUID AFTER ANTU-INDUCED EDEMA IN RATS
Autore:
GUERY BP; NELSON S; VIGET N; FIALDES P; SUMMER WR; DOBARD E; BEAUCAIRE G; MASON CM;
Indirizzi:
CTR HOSP TOURCOING,SERV REANIMAT MED & MALAD INFECT,135 RUE PDT COTY F-59208 TOURCOING FRANCE FAC MED LILLE,LAB RECH PATHOL INFECT F-59045 LILLE FRANCE FAC MED LILLE,BIOPHYS LAB F-59045 LILLE FRANCE LOUISIANA STATE UNIV,MED CTR,DEPT MED NEW ORLEANS LA 70112
Titolo Testata:
Journal of applied physiology (1985)
fascicolo: 3, volume: 85, anno: 1998,
pagine: 842 - 848
SICI:
8750-7587(1998)85:3<842:FDCIII>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; INDUCED PULMONARY-EDEMA; ACUTE LUNG INJURY; END-EXPIRATORY PRESSURE; ALPHA-NAPHTHYLTHIOUREA; TIDAL VOLUME; GUINEA-PIGS; PERMEABILITY; VENTILATION; ENDOTOXIN;
Keywords:
BRONCHOALVEOLAR LAVAGE; ALPHA-NAPHTHYLTHIOUREA; PERMEABILITY; LUNG INJURY; DEXTRAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
B.P. Guery et al., "FLUORESCEIN-LABELED DEXTRAN CONCENTRATION IS INCREASED IN BAL FLUID AFTER ANTU-INDUCED EDEMA IN RATS", Journal of applied physiology (1985), 85(3), 1998, pp. 842-848

Abstract

Several methodologies have been developed to assess alveolocapillary membrane permeability in acute lung injury. The purpose of this study was to determine the reliability of FITC-dextran compared with radioactive tracers to assess lung permeability alterations. After intraperitoneal administration of alpha-naphthylthiourea (ANTU, 50 mg/kg) or DMSO-ANTU vehicle, the animals were euthanized and their lungs were studied in an isolated-lung preparation. FITC-dextran or radiolabeled tracers were added to the perfusate. At 2 h the bronchoalveolar lavage (BAL) fluid from the ANTU group showed a significantly greater amount of fluorescence in the supernatant after centrifugation of BAL fluid compared with the DMSO group. Consistent results were observed with the radioactive tracers: there was an increase in extravascular albumin spaceand extravascular lung water compared with the control group. No cleavage of the FITC from the dextran molecule was evident by chromatography comparing samples recovered from the BAL fluid to the pure FITC-dextran molecule. In conclusion, measurement of FITC-dextran in the supernatant of BAL fluid after intravascular administration is a reliable method of assessing lung permeability changes in vivo and ex vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 17:55:11