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Titolo:
DYNAMIC EXPRESSION OF L-SELECTIN IN CELL-TO-CELL INTERACTIONS BETWEENNEUTROPHILS AND ENDOTHELIAL-CELLS IN-VITRO
Autore:
LIU Q; KISHIMOTO TK; MAINOLFI E; DELEON RP; MYERS C; MORETZ RC;
Indirizzi:
UNIV MINNESOTA,DEPT BIOL DULUTH MN 55812 BOEHRINGER INGELHEIM PHARMACEUT INC RIDGEFIELD CT 06877
Titolo Testata:
Experimental cell research
fascicolo: 1, volume: 243, anno: 1998,
pagine: 87 - 93
SICI:
0014-4827(1998)243:1<87:DEOLIC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEMOTACTIC FACTORS; POLYMORPHONUCLEAR LEUKOCYTES; TRANSENDOTHELIAL MIGRATION; SPATIAL-DISTRIBUTION; ADHESION MOLECULE-1; CYTOPLASMIC DOMAIN; MAC-1; ADHERENCE; RECEPTOR; INVIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
Q. Liu et al., "DYNAMIC EXPRESSION OF L-SELECTIN IN CELL-TO-CELL INTERACTIONS BETWEENNEUTROPHILS AND ENDOTHELIAL-CELLS IN-VITRO", Experimental cell research, 243(1), 1998, pp. 87-93

Abstract

Neutrophil-endothelial cell interactions are regulated by cell adhesion molecules and their cognate ligands. It has been proposed that L-selectin and Mac-1 (CD11b/CD18), two neutrophil adhesion receptors, havesequential roles in neutrophil extravasation during inflammation. In this model, L-selectin mediates rolling and initial adherence of neutrophils to endothelial cells, while Mac-1 strengthens this initial adherence and also facilitates migration of neutrophils through endothelial cells. L-selectin and Mac-1 expression are known to be inversely regulated. Here an in vitro culture system has been developed to investigate in situ expression of L-selectin during cell-to-cell interactions between neutrophils and endothelial cell monolayers by confocal immunofluorescence analysis. Neutrophils underwent profound cell shape change from round to polarized cell morphology with pseudopod formation after 5 to 15 min coculture with IL-1-stimulated human endothelial cells. L-selectin was redistributed to the pseudopod of the polarized neutrophils in correlation with such cellular changes. During initial cell attachment, neutrophils bound to IL-1-stimulated endothelial cells expressed a high level of L-selectin in a polarized pattern. L-selectin expression decreased over time during neutrophil-endothelial cell interactions. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:36:02