Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ANTIGENICITY OF FULLERENES - ANTIBODIES SPECIFIC FOR FULLERENES AND THEIR CHARACTERISTICS
Autore:
CHEN BX; WILSON SR; DAS M; COUGHLIN DJ; ERLANGER BF;
Indirizzi:
COLUMBIA UNIV,DEPT MICROBIOL,701 W 168 ST NEW YORK NY 10032 COLUMBIA UNIV,DEPT MICROBIOL NEW YORK NY 10032 SPHERE BIOSYST NEW BRUNSWICK NJ 08901
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 18, volume: 95, anno: 1998,
pagine: 10809 - 10813
SICI:
0027-8424(1998)95:18<10809:AOF-AS>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-60; WATER; CYCLODEXTRIN; INHIBITION; CHEMISTRY; FACILE; C60;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
B.X. Chen et al., "ANTIGENICITY OF FULLERENES - ANTIBODIES SPECIFIC FOR FULLERENES AND THEIR CHARACTERISTICS", Proceedings of the National Academy of Sciences of the United Statesof America, 95(18), 1998, pp. 10809-10813

Abstract

The recent interest in using Buckminsterfullerene (fullerene) derivatives in biological systems raises the possibility of their assay by immunological procedures. This, in turn, leads to the question of the ability of these unprecedented polygonal structures, made up solely of carbon atoms, to induce the production of specific antibodies. Immunization of mice with a C-60 fullerene derivative conjugated to bovine thyroglobulin yielded a population of fullerene-specific antibodies of the IgG isotype, showing that the immune repertoire was diverse enough to recognize and process fullerenes as protein conjugates, The population of antibodies included a subpopulation that crossreacted with a C-70 fullerene as determined by immune precipitation and ELISA procedures, These assays were made possible by the synthesis of water-soluble fullerene derivatives, including bovine and rabbit serum albumin conjugates and derivatives of trilysine and pentalysine, all of which were characterized as to the extent of substitution and their UV-Vis spectra. Possible interactions of fullerenes with the combining sites of IgG are discussed based on the physical chemistry of fullerenes and previously described protein-fullerene interactions. They remain to be confirmed by the isolation of mAbs for x-ray crystallographic studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 20:37:07