Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PALMITATE TRANSPORT AND FATTY-ACID TRANSPORTERS IN RED AND WHITE MUSCLES
Autore:
BONEN A; LUIKEN JJFP; LIU S; DYCK DJ; KIENS B; KRISTIANSEN S; TURCOTTE LP; VANDERVUSSE GJ; GLATZ JFC;
Indirizzi:
UNIV WATERLOO,DEPT KINESIOL WATERLOO ON N2L 3G1 CANADA UNIV COPENHAGEN,AUGUST KROGH INST,COPENHAGEN MUSCLE RES CTR DK-2100 COPENHAGEN DENMARK UNIV SO CALIF,DEPT EXERCISE SCI LOS ANGELES CA 90089 MAASTRICHT UNIV,CARDIOVASC RES INST MAASTRICHT,DEPT PHYSIOL NL-6200 MD MAASTRICHT NETHERLANDS
Titolo Testata:
American journal of physiology: endocrinology and metabolism
fascicolo: 3, volume: 38, anno: 1998,
pagine: 471 - 478
SICI:
0193-1849(1998)38:3<471:PTAFTI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING-PROTEIN; SKELETAL-MUSCLE; LACTATE TRANSPORT; GLUCOSE-TRANSPORT; RAT-HEART; MEMBRANE-PROTEIN; CARDIAC MYOCYTES; GIANT VESICLES; OLEATE UPTAKE; ADIPOCYTE;
Keywords:
FATTY ACID TRANSLOCASE; FATTY ACID TRANSPORT PROTEIN; PLASMA MEMBRANE-BOUND FATTY ACID BINDING PROTEIN; GIANT SARCOLEMMAL VESICLES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
A. Bonen et al., "PALMITATE TRANSPORT AND FATTY-ACID TRANSPORTERS IN RED AND WHITE MUSCLES", American journal of physiology: endocrinology and metabolism, 38(3), 1998, pp. 471-478

Abstract

We performed studies 1) to investigate the kinetics of palmitate transport into giant sarcolemmal vesicles, 2) to determine whether the transport capacity is greater in red muscles than in white muscles, and 3) to determine whether putative long-chain fatty acid (LCFA) transporters are more abundant in red than in white muscles. For these studies we used giant sarcolemmal vesicles, which contained cytoplasmic fatty acid binding protein (FABP(c)), an intravesicular fatty acid sink. Intravesicular FABP(c) concentrations were sufficiently high so as not tolimit the uptake of palmitate under conditions of maximal palmitate uptake (i.e., 4.5-fold excess in white and 31.3-fold excess in red muscle vesicles). All of the palmitate taken up was recovered as unesterified palmitate. Palmitate uptake was reduced by phloretin (-50%), sulfo-N-succinimidyl oleate (-43%), anti-plasma membrane-bound FABP (FABP(pm), -30%), trypsin (-45%), and when incubation temperature was loweredto 0 degrees C (-70%). Palmitate uptake was also reduced by excess oleate (-65%), but not by excess octanoate or by glucose. Kinetic studies showed that maximal transport was 1.8-fold greater in red vesicles than in white vesicles. The Michaelis-Menten constant in both types of vesicles was similar to 6 nM. Fatty acid transport protein mRNA and fatty acid translocase (FAT) mRNA were about fivefold greater in red muscles than in white muscles. FAT/CD36 and FABP(pm) proteins in red vesicles or in homogenates were greater than in white vesicles or homogenates (P < 0.05). These studies provide the first evidence of a protein-mediated LCFA transport system in skeletal muscle. In this tissue, palmitate transport rates are greater in red than in white muscles because more LCFA transporters are available.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:18:07