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Titolo:
ADSORPTIVE-MEDIATED ENDOCYTOSIS OF A BASIC PEPTIDE IN ENTEROCYTE-LIKECACO-2 CELLS
Autore:
SAI Y; KAJITA M; TAMAI I; WAKAMA J; WAKAMIYA T; TSUJI A;
Indirizzi:
KANAZAWA UNIV,FAC PHARMACEUT SCI,DEPT PHARMACEUT,13-1 TAKARA MACHI KANAZAWA ISHIKAWA 920093 JAPAN KANAZAWA UNIV,FAC PHARMACEUT SCI,DEPT PHARMACEUT KANAZAWA ISHIKAWA 920093 JAPAN KINKI UNIV,FAC SCI & TECHNOL,DEPT CHEM OSAKA 5778502 JAPAN
Titolo Testata:
American journal of physiology: Gastrointestinal and liver physiology
fascicolo: 3, volume: 38, anno: 1998,
pagine: 514 - 520
SICI:
0193-1857(1998)38:3<514:AEOABP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-BRAIN-BARRIER; CAPILLARY ENDOTHELIAL-CELLS; HORMONE ACTH ANALOG; TRANSPORT MECHANISM; LINE CACO-2; TRANSCYTOSIS; ABSORPTION; EBIRATIDE; RECEPTOR; INVITRO;
Keywords:
INTESTINE; TRANSPORT; MACROMOLECULAR UPTAKE; ABSORPTIVE-MEDIATED DRUG DELIVERY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
Y. Sai et al., "ADSORPTIVE-MEDIATED ENDOCYTOSIS OF A BASIC PEPTIDE IN ENTEROCYTE-LIKECACO-2 CELLS", American journal of physiology: Gastrointestinal and liver physiology, 38(3), 1998, pp. 514-520

Abstract

The internalization of a basic peptide, 001-C8 [H-MeTyr-Arg-MeArg-D-Leu-NH(CH2)(2)NH2], into enterocyte-like Caco-2 cells was evaluated. Internalization of I-125-labeled 001-C8 (I-125-001-C8) increased time dependently and reached steady state at 60 min. The steady-state internalization of I-125-001-C8 (7.24 +/- 0.41 mu l/mg protein) was temperature and concentration dependent and was significantly decreased by dansylcadaverine (500 mu M), protamine (1 mM), poly-L-lysine (1 mM), E-2078 (1 mM), and ebiratide (1 mM), whereas poly-L-glutamic acid (1 mM), tyrosine (1 mM), and glycylglycine (25 mM) were not inhibitory. Predigestion of acid mucopolysaccharides by heparinase I, heparitinase, and chondroitinase ABC also decreased the internalization. The maximal internalization, the half-saturation constant, and the nonsaturable internalization of I-125-001-C8 were 1.13 +/- 0.23 pmol/mg protein, 0.47 +/-0.43 mu M, and 3.13 +/- 0.19 mu l/mg protein, respectively. Confocal microscopy also indicated the internalization of fluorescence-derived 001-C8 [001-C8-4-nitrobenz-2-oxa-1,3-diazole (001-C8-NBD)]. Granular staining seen within the cell, excluding nuclei, indicated the sequestration of 001-C8-NBD within endocytotic vesicles. Dansylcadaverine and protamine strongly decreased the granular distribution of 001-C8-NBD within the cell. These results demonstrate that 001-C8 is taken up by Caco-2 cells via adsorptive-mediated endocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:14:04