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Titolo:
HELICOBACTER PYLORI-INFECTED HUMAN ANTRAL PRIMARY-CELL CULTURES - EFFECT ON GASTRIN CELL-FUNCTION
Autore:
RICHTERDAHLFORS A; HECZKO U; MELOCHE RM; FINLAY BB; BUCHAN AMJ;
Indirizzi:
UNIV BRITISH COLUMBIA,DEPT PHYSIOL,2146 HLTH SCI MALL VANCOUVER BC V6T 1Z3 CANADA UNIV BRITISH COLUMBIA,DEPT PHYSIOL VANCOUVER BC V6T 1Z3 CANADA UNIV BRITISH COLUMBIA,DEPT SURG VANCOUVER BC V6T 1Z3 CANADA UNIV BRITISH COLUMBIA,BIOTECHNOL LAB VANCOUVER BC V6T 1Z3 CANADA
Titolo Testata:
American journal of physiology: Gastrointestinal and liver physiology
fascicolo: 3, volume: 38, anno: 1998,
pagine: 393 - 401
SICI:
0193-1857(1998)38:3<393:HPHAPC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELLS; ACID-SECRETION; MUCOSAL SOMATOSTATIN; SERUM GASTRIN; RECEPTOR; RELEASE; ERADICATION; ADHERENCE; LINK;
Keywords:
ULTRASTRUCTURE; BACTERIAL PEDESTALS; BACTERIAL ATTACHMENT; BOMBESIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
A. Richterdahlfors et al., "HELICOBACTER PYLORI-INFECTED HUMAN ANTRAL PRIMARY-CELL CULTURES - EFFECT ON GASTRIN CELL-FUNCTION", American journal of physiology: Gastrointestinal and liver physiology, 38(3), 1998, pp. 393-401

Abstract

Although Helicobacter pylori infection increases gastrin secretion, it is unknown whether this is a direct effect or requires activation ofthe immune system. We developed an H. pylori-infected human primary antral epithelial cell culture model to address this question. This culture protocol favors growth of H. pylori, and infected cultures could be maintained for up to 48 h. These cultures were enriched for gastrin(10-40%), somatostatin (2-5%), and gastric mucin (60-80%) cells but did not contain immunocytes. Bacterial attachment occurred in a random manner within 2 h of infection, although bacterial density was lower than in sections from infected patients. After 24 or 48 h, the bacterial microcolonies were similar in size to those seen in vivo, and at 24 h ultrastructural studies demonstrated well-developed pedestal formation underlying the bacteria. Coculture with H. pylori increased basal but not stimulated gastrin secretion at all time points >2 h. In conclusion, a newly developed cell culture model has been used to characterize the interactions between H. pylori and normal human antral epithelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 12:19:57