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Titolo:
POU TRANSCRIPTION FACTORS CONTROL EXPRESSION OF CNS STEM CELL-SPECIFIC GENES
Autore:
JOSEPHSON R; MULLER T; PICKEL J; OKABE S; REYNOLDS K; TURNER PA; ZIMMER A; MCKAY RDG;
Indirizzi:
NINCDS,MOL BIOL LAB,36 CONVENT DR BETHESDA MD 20892 NINCDS,MOL BIOL LAB BETHESDA MD 20892 NIMH,DEV BIOL UNIT BETHESDA MD 20892
Titolo Testata:
Development
fascicolo: 16, volume: 125, anno: 1998,
pagine: 3087 - 3100
SICI:
0950-1991(1998)125:16<3087:PTFCEO>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; FIBROBLAST GROWTH-FACTOR; LIPID-BINDING PROTEIN; HIPPOCAMPAL-NEURONS; RETINOIC ACID; FACTOR BRN-2; FACTOR SCIP; DOMAIN; BRAIN; DIFFERENTIATION;
Keywords:
NEUROEPITHELIUM; PRECURSOR CELL; NESTIN INTERMEDIATE; FILAMENT; BRAIN FATTY ACID BINDING PROTEIN; MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
66
Recensione:
Indirizzi per estratti:
Citazione:
R. Josephson et al., "POU TRANSCRIPTION FACTORS CONTROL EXPRESSION OF CNS STEM CELL-SPECIFIC GENES", Development, 125(16), 1998, pp. 3087-3100

Abstract

Multipotential stem cells throughout the developing central nervous system have common properties. Among these is expression of the intermediate filament protein nestin and the brain fatty acid binding protein(B-FABP), To determine if common mechanisms control transcription in CNS stem cells, the regulatory elements of these two genes were mappedin transgenic mice. A 257 basepair enhancer of the rat nestin gene issufficient for expression throughout the embryonic neuroepithelium. This enhancer contains two sites bound by the class III POU proteins Brn-1, Brn-2, Brn-4, and Tst-1, Only one of the two POU sites is required for CNS expression. An adjacent hormone response element is necessary for expression in the dorsal midbrain and forebrain, The regulatory sites of the B-FABP gene are strikingly similar to those of the nestingene. A hybrid POU/Pbx binding site is recognized in vitro by Pbx-1, Brn-1 and Brn-2, This site is essential for expression in most of the CNS. In addition, a hormone response element is necessary for forebrain expression, Both the nestin and B-FABP genes therefore depend on POUbinding sites for general CNS expression, with hormone response elements additionally required for activity in the anterior CNS. These dataindicate that regulation by POU proteins and hormone receptors is a general mechanism for CNS stem cell-specific transcription.

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Documento generato il 12/07/20 alle ore 09:20:15