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Titolo:
MAMI, A HUMAN ENDOMETRIAL STROMAL SARCOMA CELL-LINE THAT CONSTITUTIVELY PRODUCES INTERLEUKIN-6, INTERLEUKIN-8, AND MONOCYTE CHEMOATTRACTANTPROTEIN-1
Autore:
NASU K; MATSUI N; NARAHARA H; TANAKA Y; TAKAI N; MIYAKAWA I; HIGUCHI Y;
Indirizzi:
OITA MED UNIV,DEPT OBSTET & GYNECOL HASAMA OITA 8795503 JAPAN OITA MED UNIV,DEPT PATHOL HASAMA OITA 8795503 JAPAN
Titolo Testata:
Archives of pathology and laboratory medicine
fascicolo: 9, volume: 122, anno: 1998,
pagine: 836 - 841
SICI:
0003-9985(1998)122:9<836:MAHESS>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MENSTRUAL-CYCLE; IN-VIVO; GENE; EXPRESSION; CULTURE; PREGNANCY; SECRETION; MOLECULES; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
K. Nasu et al., "MAMI, A HUMAN ENDOMETRIAL STROMAL SARCOMA CELL-LINE THAT CONSTITUTIVELY PRODUCES INTERLEUKIN-6, INTERLEUKIN-8, AND MONOCYTE CHEMOATTRACTANTPROTEIN-1", Archives of pathology and laboratory medicine, 122(9), 1998, pp. 836-841

Abstract

Background.-Uterine endometrial stromal sarcoma is a rare neoplasm with a morphology that closely resembles that of the proliferative endometrial stroma. To understand its pathologic characteristics, we established a novel cell line, MaMi, from a primary culture of an endometrial stromal sarcoma obtained from a 65-year-old Japanese woman. Methods.-We observed the morphology of MaMi cells and performed immunohistochemical analysis on the primary tumor and transplants in nude mice. Prolactin, insulin-like growth factor-binding protein-1, interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1, intercellular adhesion molecule-1, E-selectin, vascular cell adhesion molecule-1, and fibronectin production in the culture medium of MaMi cells were also examined. Results.-MaMi cells were shown to exhibit a fibroblastlike morphology in vitro, and they adopted a more elongated appearance in response to 12-O-tetradecanoyl phorbol-13-acetate (TPA). On injection into nude mice, the cells gave rise to subcutaneous tumors. Immunohistologically, both the primary tumor and MaMi cell-induced tumors stained positivelywith antibodies to neuron-specific enolase or vimentin. MaMi cells constitutively produced IL-6, IL-8, and monocyte chemoattractant protein-1 in vitro. Interleukin-1 beta (100 pmol/L), tumor necrosis factor-alpha (1 nmol/L), and lipopolysaccharide (1 mu g/mL) each increased the release of IL-6, IL-8, and monocyte chemoattractant protein-1 by MaMi cells. TPA (10 nmol/L) also stimulated the production of IL-6 and IL-8by these cells, but inhibited that of monocyte chemoattractant protein-1. Conclusions.-We demonstrated that MaMi cells closely resemble proliferative endometrial stromal cells not only morphologically, but also functionally. This cell line may prove valuable in understanding therole of cytokines produced by tumor cells in the pathogenesis of endometrial stromal sarcoma and may also be useful as an in vitro model offunctioning endometrial stromal cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:56:46