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Titolo:
A POPLAR TREE PROTEINASE INHIBITOR-LIKE GENE PROMOTER IS RESPONSIVE TO WOUNDING IN TRANSGENIC TOBACCO
Autore:
HOLLICK JB; GORDON MP;
Indirizzi:
UNIV WASHINGTON,DEPT BIOCHEM SJ 70 SEATTLE WA 98195 UNIV WASHINGTON,DEPT BIOCHEM SJ 70 SEATTLE WA 98195
Titolo Testata:
Plant molecular biology
fascicolo: 4, volume: 22, anno: 1993,
pagine: 561 - 572
SICI:
0167-4412(1993)22:4<561:APTPIG>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
REACTIVE CENTER REGIONS; II GENE; EVOLUTIONARY DIVERGENCE; MEDIATED TRANSFORMATION; PROTEOLYTIC-ENZYMES; PROTEASE INHIBITORS; TRYPSIN-INHIBITORS; BETA-GLUCURONIDASE; PLANTS; POTATO;
Keywords:
GUS; POPULUS; COTTONWOOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
J.B. Hollick e M.P. Gordon, "A POPLAR TREE PROTEINASE INHIBITOR-LIKE GENE PROMOTER IS RESPONSIVE TO WOUNDING IN TRANSGENIC TOBACCO", Plant molecular biology, 22(4), 1993, pp. 561-572

Abstract

Wounding of poplar trees leads to the accumulation of several mRNA species that encode proteins with putative defensive function. One classof wound-induced poplar RNA (win3) has amino acid sequence similarityto Kunitz-type trypsin inhibitors. Northern blots and cDNA sequencingshow that several win3 mRNAs accumulate in the uninjured leaves of wounded trees. We report further characterization of the win3 family including sequence comparisons, gene family organization, and the identification of one win3 member that is transcriptionally activated in response to mechanical wounding. We also show that 1.5 kb of 5'-flanking sequence of one win3 member (win3.12) is sufficient to confer wound-regulated expression of a beta-D-glucuronidase (GUS) reporter gene in transgenic tobacco. Annual herbaceous plants such as tobacco can thus be used to study the expression of genes from a perennial woody angiosperm.

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Documento generato il 04/04/20 alle ore 14:58:04