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Titolo:
DILUTION OF N-15 IN DUNALIELLA-TERTIOLECTA BY UPTAKE OF AN UNIDENTIFIED COMPOUND FOLLOWING NITRATE EXHAUSTION
Autore:
COLLOS Y; SCIANDRA A; MARTINJEZEQUEL V; CUEFF A; SLAWYK G; GARCIA N;
Indirizzi:
UNIV MONTPELLIER 2,LAB HYDROBIOL,UMR CNRS 5556,CC093 F-34095 MONTPELLIER 5 FRANCE LEPM,STN ZOOL,URA CNRS 2077 F-06230 VILLEFRANCHE MER FRANCE CNRS,BIOL STN,UPR 4601 F-29680 ROSCOFF FRANCE CNRS,UMR 6535,CTR OCEANOL F-13288 MARSEILLE 9 FRANCE
Titolo Testata:
Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie
fascicolo: 8, volume: 321, anno: 1998,
pagine: 673 - 677
SICI:
0764-4469(1998)321:8<673:DONIDB>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
DISSOLVED ORGANIC NITROGEN; DIATOM SKELETONEMA-COSTATUM; MARINE-PHYTOPLANKTON; TRACER METHOD; AMINO-ACIDS; RELEASE; NITRITE; ASSIMILATION; PACIFIC; CULTURE;
Keywords:
NITROGEN BUDGET; NITRATE UPTAKE; NEW PRODUCTION; N-15 DILUTION; DUNALIELLA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
Y. Collos et al., "DILUTION OF N-15 IN DUNALIELLA-TERTIOLECTA BY UPTAKE OF AN UNIDENTIFIED COMPOUND FOLLOWING NITRATE EXHAUSTION", Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie, 321(8), 1998, pp. 673-677

Abstract

Nitrate (about 20 mu M) was added as (NO3)-N-15 to a nitrate-limited continuous culture of Dunaliella tertiolecta at steady-state. Nitrate uptake was then estimated from the decrease in nitrate in the medium, the incorporation of N-15 into cells, and the increase in cellular nitrogen. Although the overall nitrogen budget over 5 h was balanced, there were large differences in estimates (up to a factor of five) of nitrate assimilation by the three methods on shorter time scale. After nitrate was exhausted from the medium, cellular nitrogen continued to increase while the N-15 content of the particulate matter decreased overthe next 1.5 h. This indicated that an unidentified, unlabelled nitrogen form, which was neither nitrite, ammonium nor dissolved free aminoacids, was being taken up by the cells, at rates comparable to those of nitrate. This phenomenon leads to an underestimation of new biomassproduction when assessed through N-15 incorporation into cells. ((C) Academie des sciences / Elsevier, Paris.).

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Documento generato il 22/09/20 alle ore 20:08:33