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Titolo:
CLONING, EXPRESSION, CHARACTERIZATION AND ROLE OF THE LEADER SEQUENCEOF A LIPASE FROM RHIZOPUS-ORYZAE
Autore:
BEER HD; MCCARTHY JEG; BORNSCHEUER UT; SCHMID RD;
Indirizzi:
UNIV STUTTGART,INST TECH BIOCHEM,ALLMANDRING 31 D-70569 STUTTGART GERMANY UNIV STUTTGART,INST TECH BIOCHEM D-70569 STUTTGART GERMANY MPI BIOCHEM,AG WERNER D-82152 MARTINSRIED GERMANY UMIST,DEPT BIOCHEM MANCHESTER M60 1QD LANCS ENGLAND
Titolo Testata:
Biochimica et biophysica acta, N. Gene structure and expression
fascicolo: 2-3, volume: 1399, anno: 1998,
pagine: 173 - 180
SICI:
0167-4781(1998)1399:2-3<173:CECARO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; PROTEIN TRANSLOCATION; FILAMENTOUS FUNGI; PURIFICATION; GENE; CRYSTALLIZATION; DELEMAR; NIVEUS; EXPORT; RNA;
Keywords:
LIPASE; LEADER SEQUENCE; CLONING; EXPRESSION; SEQUENCE; (RHIZOPUS ORYZAE);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
H.D. Beer et al., "CLONING, EXPRESSION, CHARACTERIZATION AND ROLE OF THE LEADER SEQUENCEOF A LIPASE FROM RHIZOPUS-ORYZAE", Biochimica et biophysica acta, N. Gene structure and expression, 1399(2-3), 1998, pp. 173-180

Abstract

A lipase from Rhizopus oryzae DSM 853 (ROL) was cloned from a chromosomal gene bank, sequenced and overexpressed in Escherichia coli. ROL and its precursors ProROL and PreProROL were purified and their pH and temperature profile was determined. In contrast to ROL, ProROL and PreProROL had considerably higher thermostability and a slightly higher pH optimum. Moreover, it could be demonstrated by in vitro experiments that the natural leader sequence of ROL is able to inhibit the foldingsupporting properties of the prosequence, resulting in a retardation of folding. In addition, there is strong evidence that all different lipase forms derived from Rhizopus sp. described in the literature are a result of different proteolytic processing and originate from the same gene. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:36:13