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Titolo:
ANTAGONIST INHIBITION OF GABA-A-MEDIATED CHLORIDE UPTAKE IS ATYPICAL IN NIGROCOLLICULAR AREAS
Autore:
PERIS J;
Indirizzi:
UNIV FLORIDA,HLTH SCI CTR,DEPT PHARMACODYNAM,BOX 100487 GAINESVILLE FL 32610
Titolo Testata:
Life sciences
fascicolo: 9, volume: 53, anno: 1993,
pagine: 707 - 715
SICI:
0024-3205(1993)53:9<707:AIOGCU>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; SUBUNIT MESSENGER-RNAS; CENTRAL-NERVOUS-SYSTEM; A RECEPTOR SUBTYPES; S-35 TBPS BINDING; RAT-BRAIN; FUNCTIONAL EXPRESSION; AUTORADIOGRAPHIC LOCALIZATION; BENZODIAZEPINE PHARMACOLOGY; MOLECULAR-BIOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
J. Peris, "ANTAGONIST INHIBITION OF GABA-A-MEDIATED CHLORIDE UPTAKE IS ATYPICAL IN NIGROCOLLICULAR AREAS", Life sciences, 53(9), 1993, pp. 707-715

Abstract

Marked regional differences in the mRNA levels for the different subunits of the GABA(A) receptor may result in functional differences throughout the brain. The effects of different GABA(A) agonists and antagonists on GABA(A) receptor function was measured using Cl-36- uptake inmicrosacs made from cerebellum (CB), superior colliculus (SC), substantia nigra (SN), inferior colliculus (IC) or cortex (CT) containing equal amounts of protein. GABA increased Cl- uptake in all regions in a dose-dependent and saturable manner but with markedly different efficacies across brain regions. Maximal GABA-stimulated uptake was 59 +/- 2nmoles of Cl- per mg protein in CT, 25 +/- 5 in SC, 24 +/- 4 in CB, 16 +/- 1 in IC and 6.7 +/- 1.4 in SN. Muscimol increased Cl- uptake with greater potency than GABA but with similar efficacies in CT, CB, SC and SN; the efficacy of muscimol in IC was increased to 27.4 +/- 5. Picrotoxin inhibited maximal GABA-stimulated Cl- uptake in CB, SC and CTbut did not decrease uptake in IC or SN. Bicuculline methiodide completely inhibited uptake in CB and CT, partially inhibited uptake in IC,and did not affect uptake in SC and SN. These data provide functionalsupport for regional heterogeneity of the GABA(A) receptor/Cl-ionophore between nigrocollicular regions and CB and CT.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/05/20 alle ore 15:46:08