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Titolo:
DIMERIC AND TETRAMERIC FORMS OF CATALYTICALLY ACTIVE TRANSMEMBRANE CD38 IN TRANSFECTED HELA-CELLS
Autore:
BRUZZONE S; GUIDA L; FRANCO L; ZOCCHI E; CORTE G; DEFLORA A;
Indirizzi:
UNIV GENOA,INST BIOCHEM,VIALE BENEDETTO XV,I I-16132 GENOA ITALY UNIV GENOA,INST BIOCHEM I-16132 GENOA ITALY UNIV GENOA,NATL INST CANC RES CBA GENOA ITALY UNIV GENOA,DEPT CLIN & EXPT ONCOL GENOA ITALY
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 433, anno: 1998,
pagine: 275 - 278
SICI:
0014-5793(1998)433:3<275:DATFOC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADP-RIBOSYL CYCLASE; IDENTIFICATION; GLYCOHYDROLASE; HYDROLYSIS;
Keywords:
CD38; CYCLIC ADP-RIBOSE; CD38 OLIGOMER; ECTOENZYME; NAD(+);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
S. Bruzzone et al., "DIMERIC AND TETRAMERIC FORMS OF CATALYTICALLY ACTIVE TRANSMEMBRANE CD38 IN TRANSFECTED HELA-CELLS", FEBS letters, 433(3), 1998, pp. 275-278

Abstract

CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclicADP-ribose (cADPR) from NAD(+) and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S,, Zocchi, E,, Usai, C, and De Flora, A. (1998) FASEB J, in press]. cADPR regulates intracellular calcium homeostasis by releasing calcium from responsive stores. The cADPR-transporting function of CD38 requires channel-generating oligomeric forms of the protein rather than the 46 kDa monomers that have been described so far in CD38(+) cells. Here me demonstrate that CD38, both in reconstituted proteoliposomes and in CD38-transfected HeLa cells, is a mixture of catalytically active monomers, homodimers andhomotetramers, A soluble recombinant form of CD38 corresponding to its ectocellular region proved to be monomeric, Thus, association of native CD38 with either artificial or natural membranes seems to result in a reversible juxtaposition of monomers suitable to cADPR-transporting activity, (C) 1998 Federation of European Biochemical Societies.

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Documento generato il 18/09/20 alle ore 20:07:37