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Titolo:
PCR-BASED DETECTION OF XANTHOMONAS-CAMPESTRIS PV-PHASEOLI VAR FUSCANSIN PLANT-MATERIAL AND ITS DIFFERENTIATION FROM X-C-PV-PHASEOLI
Autore:
TOTH IK; HYMAN LJ; TAYLOR R; BIRCH PRJ;
Indirizzi:
SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND HORT & FOOD RES INST NEW ZEALAND LTD,MT ALBERT RES CTR AUCKLAND NEW ZEALAND
Titolo Testata:
Journal of applied microbiology
fascicolo: 2, volume: 85, anno: 1998,
pagine: 327 - 336
SICI:
1364-5072(1998)85:2<327:PDOXPV>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CAROTOVORA SUBSP ATROSEPTICA; SENSITIVE DETECTION; PSEUDOMONAS-SOLANACEARUM; OLIGONUCLEOTIDE PRIMERS; PATHOGENIC VARIATION; BACTERIAL-BLIGHT; DNA-SEQUENCES; CAUSAL AGENT; COMMON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
I.K. Toth et al., "PCR-BASED DETECTION OF XANTHOMONAS-CAMPESTRIS PV-PHASEOLI VAR FUSCANSIN PLANT-MATERIAL AND ITS DIFFERENTIATION FROM X-C-PV-PHASEOLI", Journal of applied microbiology, 85(2), 1998, pp. 327-336

Abstract

A PCR-based method was developed for the specific detection of Xanthomonas campestris pv. phaseoli var. fuscans from plant material. Primers Xf1 and Xf2, based on a sequence conserved amplified region (SCAR) derived from RAPD PCR analysis of X. c. pv. phaseoli var.fuscans, amplified a DNA fragment of 450 bp from all such isolates. In contrast, no amplification product was obtained from any X. c. pv, phaseoli isolates, or from any other DNAs tested. As few as 10 cells of X. c. pv. phaseoli var. fuscans (equivalent to about 100 fg DNA) could be detected in vitro. In planta, following an initial inoculation of as little as one cell, an amplification product was generated after only 2 d of incubation, allowing highly sensitive detection 10 d before disease symptoms were observed. Moreover, the failure to amplify DNA from X. c. pv. phaseoli isolates shows that these primers provide a rapid, improved method to differentiate these two varieties using PCR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:45:48