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Titolo:
NEURONAL CA2-1, THE MAMMALIAN HOMOLOG OF FREQUENIN, IS EXPRESSED IN CHROMAFFIN AND PC12 CELLS AND REGULATES NEUROSECRETION FROM DENSE-CORE GRANULES( SENSOR)
Autore:
MCFERRAN BW; GRAHAM ME; BURGOYNE RD;
Indirizzi:
UNIV LIVERPOOL,PHYSIOL LAB,CROWN ST LIVERPOOL L69 3BX MERSEYSIDE ENGLAND UNIV LIVERPOOL,PHYSIOL LAB LIVERPOOL L69 3BX MERSEYSIDE ENGLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 35, volume: 273, anno: 1998,
pagine: 22768 - 22772
SICI:
0021-9258(1998)273:35<22768:NCTMHO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-BINDING PROTEIN; NERVOUS-SYSTEM; CA2+-DEPENDENT EXOCYTOSIS; TRANSMITTER RELEASE; MOLECULAR-CLONING; DROSOPHILA; NEUROCALCIN; RECOVERIN; HIPPOCALCIN; SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
B.W. Mcferran et al., "NEURONAL CA2-1, THE MAMMALIAN HOMOLOG OF FREQUENIN, IS EXPRESSED IN CHROMAFFIN AND PC12 CELLS AND REGULATES NEUROSECRETION FROM DENSE-CORE GRANULES( SENSOR)", The Journal of biological chemistry, 273(35), 1998, pp. 22768-22772

Abstract

Neuronal Ca2+ sensor 1 (NCS-1) is the mammalian homologue of the Ca2+-binding protein frequenin previously implicated in regulation of neurotransmission in Drosophila (Pongs, O., Lindemeier, J., Zhu, X. R., Theil, T., Endelkamp, D., Krah-Jentgens, I., Lambrecht, H.-G., Koch, R. W., Schwemer, J., Rivosecchi, R., Mallart, A., Galceran, J., Canal, I., Barbas, J. A., and Ferrus, A. (1993) Neuron 11, 15-28). NCS-1 has been considered to be expressed only in neurons, but we show that NCS-1 expression can be detected in bovine adrenal chromaffin and PC12 cells, two widely studied model neuroendocrine cells. NCS-1 was present in both cytosolic and membrane fractions including purified chromaffin granules, and in immunofluorescence, its distribution overlapped with peripheral punctate staining seen with the synaptic-like microvesicle marker synaptophysin in PC12 cells. The possible functional role of NCS-1 in exocytosis of dense core granules was tested using transient transfection in PC12 cells and assay of co-transfected growth hormone (GH)release. Overexpression of NCS-1 increased evoked GH release in intact cells in response to ATP. No effect of overexpression was seen on GHrelease because of Ca2+ in permeabilized cells suggesting that NCS-1 may have a regulatory but not direct role in neurosecretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 00:19:25