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Titolo:
INVOLVEMENT OF PHOSPHOLIPASE C-GAMMA-1 IN MOUSE EGG ACTIVATION-INDUCED BY A TRUNCATED FORM OF THE C-KIT TYROSINE KINASE PRESENT IN SPERMATOZOA
Autore:
SETTE C; BEVILACQUA A; GEREMIA R; ROSSI P;
Indirizzi:
UNIV ROMA TOR VERGATA,DIPARTIMENTO SANITA PUBBL & BIOL CELLULARE,SEZ ANAT,VIA O RAIMONDO 8 I-00173 ROME ITALY UNIV ROMA TOR VERGATA,DIPARTIMENTO SANITA PUBBL & BIOL CELLULARE,SEZ ANAT I-00173 ROME ITALY UNIV ROMA LA SAPIENZA,DIPARTIMENTO ISTOL & EMBRIOL MED ROME ITALY UNIV ROMA LA SAPIENZA,DIPARTIMENTO PSICOL ROME ITALY
Titolo Testata:
The Journal of cell biology
fascicolo: 4, volume: 142, anno: 1998,
pagine: 1063 - 1074
SICI:
0021-9525(1998)142:4<1063:IOPCIM>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRIGGERS CALCIUM OSCILLATIONS; SEA-URCHIN EGG; STEM-CELL; SIGNAL-TRANSDUCTION; SH3 DOMAIN; MEIOTIC MATURATION; ARACHIDONIC-ACID; MAMMALIAN EGGS; HAMSTER EGGS; CA2+ WAVE;
Keywords:
TRUNCATED C-KIT; SPERMATOZOA; EGG ACTIVATION; PHOSPHOLIPASE-C-GAMMA-1; SRC-HOMOLOGY DOMAINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
72
Recensione:
Indirizzi per estratti:
Citazione:
C. Sette et al., "INVOLVEMENT OF PHOSPHOLIPASE C-GAMMA-1 IN MOUSE EGG ACTIVATION-INDUCED BY A TRUNCATED FORM OF THE C-KIT TYROSINE KINASE PRESENT IN SPERMATOZOA", The Journal of cell biology, 142(4), 1998, pp. 1063-1074

Abstract

Microinjection of a truncated form of the c-kit tyrosine kinase present in mouse spermatozoa (tr-kit) activates mouse eggs parthenogenetically, and tr-kit-induced egg activation is inhibited by preincubation with an inhibitor of phospholipase C (PLC) (Sette, C., A. Bevilacqua, A. Bianchini, F. Mangia, R, Geremia, and F. Rossi. 1997. Development [Camb.]. 124:2267-2274). Coinjection of glutathione-S-transferase (GST) fusion proteins containing the src-homology (SH) domains of the gamma 1 isoform of PLC (PLC gamma 1) competitively inhibits tr-kit-induced egg activation. A GST fusion protein containing the SH3 domain of PLC gamma 1 inhibits egg activation as efficiently as the whole SH region, while a GST fusion protein containing the two SH2 domains is much lesseffective. A GST fusion protein containing the SH3 domain of the Grb2adaptor protein does not inhibit tr-kit-induced egg activation, showing that the effect of the SH3 domain of PLC gamma 1 is specific. Tr-kit-induced egg activation is also suppressed by co-injection of antibodies raised against the PLC gamma 1 SH domains, but not against the PLCy1 COOH-terminal region. In transfected COS cells, coexpression of PLCgamma 1 and tr-kit increases diacylglycerol and inositol phosphate production, and the phosphotyrosine content of PLC gamma 1 with respect to cells expressing PLC gamma 1 alone. These data indicate that tr-kitactivates PLCy1, and that the SH3 domain of PLC gamma 1 is essential for tr-kit-induced egg activation.

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Documento generato il 28/11/20 alle ore 00:33:10