Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ERYTHEMA MULTIFORME ASSOCIATED HUMAN AUTOANTIBODIES AGAINST DESMOPLAKIN-I AND DESMOPLAKIN-II - BIOCHEMICAL-CHARACTERIZATION AND PASSIVE TRANSFER STUDIES INTO NEWBORN MICE
Autore:
FOEDINGER D; ELBEBURGER A; STERNICZKY B; LACKNER M; HORVAT R; WOLFF K; RAPPERSBERGER K;
Indirizzi:
UNIV VIENNA,SCH MED,DEPT DERMATOL,DIV GEN DERMATOL,WAHRINGER GURTEL 18-20 A-1090 VIENNA AUSTRIA VIENNA INT RES COOPERAT CTR,DIV GEN DERMATOL,DEPT DERMATOL VIENNA AUSTRIA VIENNA INT RES COOPERAT CTR,DIV IMMUNOL ALLERGY & INFECT DIS VIENNA AUSTRIA UNIV VIENNA,SCH MED,DEPT CLIN PATHOL VIENNA AUSTRIA
Titolo Testata:
Journal of investigative dermatology
fascicolo: 3, volume: 111, anno: 1998,
pagine: 503 - 510
SICI:
0022-202X(1998)111:3<503:EMAHAA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENT NETWORKS; DESMOSOMAL PLAQUE; ADHESION MOLECULES; CARBOXYL TERMINUS; EPITHELIAL-CELLS; SKIN-DISEASE; KERATIN; PEMPHIGUS; ANTIBODIES; ANTIGEN;
Keywords:
AUTOIMMUNITY; EPITOPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
D. Foedinger et al., "ERYTHEMA MULTIFORME ASSOCIATED HUMAN AUTOANTIBODIES AGAINST DESMOPLAKIN-I AND DESMOPLAKIN-II - BIOCHEMICAL-CHARACTERIZATION AND PASSIVE TRANSFER STUDIES INTO NEWBORN MICE", Journal of investigative dermatology, 111(3), 1998, pp. 503-510

Abstract

The demonstration of circulating autoantibodies directed against the constitutive desmosomal plaque proteins desmoplakin (dp) I and II in mucocutaneous lesions in a subset of patients with erythema multiforme major, suggests that humoral immune mechanisms may play a role in the pathogenesis of this severe skin disease. In this study we identified a specific peptide sequence YSYSYS - representing an antigenic bindingsite for the human autoantibodies. This epitope is localized at the extreme carboxy terminal domain of dp thought to be responsible for theassembly of keratin filaments with desmosomes. To test the possibility whether these antibodies may exert any pathologic effects in vivo, human autoantibodies were affinity purified on a corresponding synthetic peptide matrix and peptide-specific antibodies were raised in rabbits. After repeated subcutaneous injections into newborn mice, affinity-purified human autoantibodies and anti-peptide rabbit IgG were detected on desmosomal plaques of keratinocytes overlying the injection site. Histologic and electron microscopic examinations showed hydropic degeneration of basal and suprabasal keratinocytes, dyskeratosis, signs ofsuprabasal acantholysis, and keratin filaments detached from the desmosomal plaques clumping around the nucleus. We demonstrate that autoantibodies are directed to an epitope within a dp domain crucial for theinteraction of keratin filaments with desmosomes, and, when injected subcutaneously into newborn mice, produce pathologic changes. These findings imply that autoantibodies to dp could impair the function of desmosome - keratin filament complexes suggesting a pathogenic role in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 04:29:12