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Titolo:
CLONING AND CHARACTERIZATION OF PROS45, THE DROSOPHILA SUG1 PROTEASOME SUBUNIT HOMOLOG
Autore:
CHENG L; ROEMER N; SMYTH KA; BELOTE J; NAMBU JR; SCHWARTZ LM;
Indirizzi:
UNIV MASSACHUSETTS,DEPT BIOL AMHERST MA 01003 UNIV MASSACHUSETTS,DEPT BIOL AMHERST MA 01003 UNIV MASSACHUSETTS,PROGRAM MOL & CELLULAR BIOL AMHERST MA 01003 SYRACUSE UNIV,DEPT BIOL SYRACUSE NY 13244
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 1, volume: 259, anno: 1998,
pagine: 13 - 20
SICI:
0026-8925(1998)259:1<13:CACOPT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; 26S PROTEASOME; TRANSCRIPTIONAL MEDIATOR; BINDING PROTEIN; NERVOUS-SYSTEM; CAD FAMILY; YEAST; GENE; IDENTIFICATION; ACTIVATION;
Keywords:
PROTEASOME; APOPTOSIS; SUG-1; DROSOPHILA MELANOGASTER; NERVOUS SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
L. Cheng et al., "CLONING AND CHARACTERIZATION OF PROS45, THE DROSOPHILA SUG1 PROTEASOME SUBUNIT HOMOLOG", MGG. Molecular & general genetics, 259(1), 1998, pp. 13-20

Abstract

The proteasome plays essential roles in a variety of cellular processes, including degradation of the bulk of cellular proteins, degradation of short-lived proteins such as cell cycle regulators, generation ofantigenic peptides, and mediating programmed cell death. One of the best characterized subunits of the 26S proteasome is encoded by the yeast gene SUGI. We report here the cloning and characterization of the Drosophila homolog of this gene, Pros45. At the protein level, Pros45 is highly conserved with respect to its homologs in a variety of taxa: it shows 74% identity to yeast Sug1; 86% to mouse m56/mSug1/FZA-B; 87%to human Trip1; and 97% to moth 18-56. Using a genomic clone as a probe for in situ hyridization to polytene chromesomes, we demonstrated that Pros45 maps to 19F, near the base of the X chromosome. Use of a pros45 cDNA clone as a probe revealed a second site of hybridization at 99CD. Pros45 mRNA is found in the unfertilized egg and in all cells ofthe early embryo. By the end of embryogenesis, Pros45 is expressed predominantly in the central nervous system. Targeted expression of Pros45 in a variety of different cells using the Gal4 UAS P-element systemfailed to generate an overt phenotype. This study provides the foundation for further examination of the role of the 26S proteasome in homeostasis and development in Drosophila.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 20:13:53