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Titolo:
A CHIMERIC SPERM PEPTIDE INDUCES ANTIBODIES AND STRAIN-SPECIFIC REVERSIBLE INFERTILITY IN MICE
Autore:
LEA IA; VANLIEROP MJC; WIDGREN EE; GROOTENHUIS A; WEN Y; VANDUIN M; ORAND MG;
Indirizzi:
UNIV N CAROLINA,DEPT CELL BIOL & ANAT,CB 7090,210 TAYLOR HALL CHAPEL HILL NC 27599 UNIV N CAROLINA,DEPT CELL BIOL & ANAT CHAPEL HILL NC 27599 NV ORGANON,DEPT PHARMACOL NL-5340 BH OSS NETHERLANDS
Titolo Testata:
Biology of reproduction
fascicolo: 3, volume: 59, anno: 1998,
pagine: 527 - 536
SICI:
0006-3363(1998)59:3<527:ACSPIA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
B-CELL EPITOPE; SUCCESSFUL PREGNANCY; PROTEIN; IMMUNIZATION; SEQUENCE; ANTIGEN; RABBIT; SP17; IDENTIFICATION; FERTILIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
I.A. Lea et al., "A CHIMERIC SPERM PEPTIDE INDUCES ANTIBODIES AND STRAIN-SPECIFIC REVERSIBLE INFERTILITY IN MICE", Biology of reproduction, 59(3), 1998, pp. 527-536

Abstract

The development of a contraceptive vaccine based on a gamete-specificantigen requires knowledge of the ability of the antigen to elicit animmune response that inhibits fertilization. A well-defined immune response, as elicited by a synthetic peptide comprising a dominant B-cell epitope coupled to a common promiscuous T-cell epitope, might be preferable. In this study, the immunodominant B-cell epitope of sperm antigen Sp17 has been identified and synthesized as a chimeric peptide with the promiscuous T-cell epitope bovine RNase[94-104] at the N terminal. Immunization of female BALB/c mice with this peptide induced a dose-dependent reduction in fertility. Although antibodies to recombinantand native Sp17 were elicited in these mice, there was no strict correlation between the level of these antibodies and the reduction in fertility. Moreover, the induction of infertility was strain-specific since no effect on fertility could be induced in B6AF1 mice. To understand the mechanism behind this apparent strain-specific infertility induction, a more extended study on both the humoral and the cellular immune response to the chimeric peptide was performed. The antigen-specificT-cell response and the levels of antigen-specific cytokines are the major factors that affect fertility outcome.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:30:54