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Titolo:
ASSESSMENT OF D-GLUCOSE TRANSPORT KINETICS IN THE PERFUSED HUMAN PLACENTA - AN IN-VITRO STUDY
Autore:
NANDAKUMARAN M; DEV BR; MAKHSEED M; SUGATHAN TN;
Indirizzi:
KUWAIT UNIV,FAC MED,DEPT OBSTET & GYNAECOL,PP BOX 24923 SAFAT 13110 KUWAIT KUWAIT UNIV,FAC MED,DEPT PHYSIOL SAFAT 13110 KUWAIT KUWAIT UNIV,FAC MED,DEPT COMMUNITY MED SAFAT 13110 KUWAIT
Titolo Testata:
Acta Paediatrica Japonica Overseas Edition
fascicolo: 4, volume: 40, anno: 1998,
pagine: 307 - 312
SICI:
0374-5600(1998)40:4<307:AODTKI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Keywords:
D-GLUCOSE; HUMAN PLACENTA; MATERNAL-FETAL EXCHANGE; PERFUSION; TRANSPORT KINETICS; TRITIATED WATER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
M. Nandakumaran et al., "ASSESSMENT OF D-GLUCOSE TRANSPORT KINETICS IN THE PERFUSED HUMAN PLACENTA - AN IN-VITRO STUDY", Acta Paediatrica Japonica Overseas Edition, 40(4), 1998, pp. 307-312

Abstract

Background: There have been no reports to date on glucose transport kinetics and the effect of graded hyperglycemia in the human placenta in non-steady-state conditions. Methods: The transport kinetics of D-glucose in the human placenta was investigated in non-steady state conditions in vitro using perfusion of isolated placental lobules. NationalCancer Tissue Culture (NCTC) 135 culture medium, diluted with Earle'sbuffered salt solution was used as the perfusate. C-14-labeled D-glucose and water as reference were injected as a single bolus into the maternal arterial perfusate. Perfusate samples were collected and analyzed from the maternal and fetal venous outflows. Results: In four successful perfusions, differential transport rates of glucose in the maternal-fetal direction averaged 1.03, 1.02, 1.09, 1.04 and 1.03 times those of corresponding tritiated water transport rates for 10, 25, 50, 75and 90% of efflux fractions, respectively. Glucose transport fraction, expressed as percentage of injected maternal dose averaged 84 +/- 3.1% of water transport fraction in the four perfusions. Glucose kineticparameters expressed as area under the curve, elimination constant (Kel), clearance, time for maximum response, absorption rate and elimination rate averaged 0.25, 0.29, 3.96, 1.02, 0.25 and 0.18 times that ofthe corresponding tritiated water value, respectively. Neither the different kinetic parameters nor the transport fraction indices differedsignificantly when glucose concentrations in the same perfusions wereraised successively from 5.56 to 27.80 and then to 55.6 mmol/L. Conclusions: We speculate that within physiological limits, hyperglycemia per se plays no significant part in altering glucose transport kineticsacross the human placenta in the maternal-fetal direction.

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Documento generato il 17/01/21 alle ore 18:33:25