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Titolo:
HYDRODYNAMIC SHEAR SHOWS DISTINCT ROLES FOR LFA-1 AND MAC-1 IN NEUTROPHIL ADHESION TO INTERCELLULAR-ADHESION MOLECULE-1
Autore:
NEELAMEGHAM S; TAYLOR AD; BURNS AR; SMITH CW; SIMON SI;
Indirizzi:
BAYLOR COLL MED,SECT LEUKOCYTE BIOL,1100 BATES,ROOM 6014 HOUSTON TX 77030 BAYLOR COLL MED,SECT LEUKOCYTE BIOL HOUSTON TX 77030 RICE UNIV,INST BIOSCI & BIOENGN HOUSTON TX 77251 SUNY BUFFALO,DEPT CHEM ENGN BUFFALO NY 14260
Titolo Testata:
Blood
fascicolo: 5, volume: 92, anno: 1998,
pagine: 1626 - 1638
SICI:
0006-4971(1998)92:5<1626:HSSDRF>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATED ENDOTHELIAL-CELLS; L-SELECTIN; CHEMOTACTIC FACTORS; LEUKOCYTE ADHESION; INTEGRIN MAC-1; TRANSENDOTHELIAL MIGRATION; CULTURED ENDOTHELIUM; LYMPHOCYTE ADHESION; PHYSIOLOGICAL FLOW; ICAM-1 CD54;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
S. Neelamegham et al., "HYDRODYNAMIC SHEAR SHOWS DISTINCT ROLES FOR LFA-1 AND MAC-1 IN NEUTROPHIL ADHESION TO INTERCELLULAR-ADHESION MOLECULE-1", Blood, 92(5), 1998, pp. 1626-1638

Abstract

The binding of neutrophil beta(2) integrin to intercellular adhesion molecule-1 (ICAM-1) expressed on the inflamed endothelium is critical for neutrophil arrest at sites of tissue inflammation. To quantify thestrength and kinetics of this interaction, we measured the adhesion between chemotactically stimulated neutrophils and ICAM-1-transfected mouse cells (E3-ICAM) in suspension in a cone-plate viscometer at shearrates typical of venular blood flow (100 s(-1) to 500 s(-1)). The kinetics of aggregation were fit with a mathematical model based on two-body collision theory, This enabled estimation of adhesion efficiency, defined as the probability with which collisions between cells resulted in firm adhesion. The efficiency of beta(2)-integrin-dependent adhesion was highest (similar to 0.2) at 100 s(-1) and it decreased to approximately zero at 400 s(-1) Both LFA-1 and Mac-1 contributed equally to adhesion efficiency over the initial 30 seconds of stimulation, but adhesion was entirely Mac-1-dependent by 120 seconds. Two hydrodynamicparameters were observed to influence integrin-dependent adhesion efficiency: the level of shear stress and the intercellular contact duration. Below a critical shear stress (<2 dyn/cm(2)), contact duration predominantly limited adhesion efficiency. The estimated minimum contactduration for beta(2)integrin binding was approximately 6.5 ms. Above the critical shear stress (>2 dyn/cm(2)), the efficiency of neutrophiladhesion to E3-ICAM was limited by both the contact duration and the tensile stress. We conclude that at low shear, neutrophil adhesion is modulated independently through either LFA-1 or Mac-1, which initiallycontribute with equal efficiency, but differ over the duration of chemotactic stimulation. (C) 1998 by The American Society of Hematology.

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Documento generato il 18/01/21 alle ore 16:09:18