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Titolo:
DNA FRAGMENTATION, DNA-PROTEIN CROSS-LINKS, P-32 POSTLABELED NUCLEOTIDIC MODIFICATIONS, AND 8-HYDROXY-2'-DEOXYGUANOSINE IN THE LUNG BUT NOTIN THE LIVER OF RATS RECEIVING INTRATRACHEAL INSTILLATIONS OF CHROMIUM(VI) - CHEMOPREVENTION BY ORAL N-ACETYLCYSTEINE
Autore:
IZZOTTI A; BAGNASCO M; CAMOIRANO A; ORLANDO M; DEFLORA S;
Indirizzi:
UNIV GENOA,INST HYG & PREVENT MED,VIA PASTORE 1 I-16132 GENOA ITALY UNIV GENOA,INST HYG & PREVENT MED I-16132 GENOA ITALY
Titolo Testata:
Mutation research. Fundamental and molecular mechanisms of mutagenesis
fascicolo: 1-2, volume: 400, anno: 1998,
pagine: 233 - 244
SICI:
1386-1964(1998)400:1-2<233:DFDCPP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALVEOLAR MACROPHAGES; METABOLIC REDUCTION; HYDROGEN-PEROXIDE; STRAND BREAKS; IN-VITRO; GLUTATHIONE; ADDUCTS; DAMAGE; ASSAY; CARCINOGENICITY;
Keywords:
CHROMIUM(VI); DNA FRAGMENTATION; DNA-PROTEIN CROSS-LINK; NUCLEOTIDIC MODIFICATION; 8-HYDROXY-2'-DEOXYGUANOSINE; P-32 POSTLABELING; N-ACETYLCYSTEINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
A. Izzotti et al., "DNA FRAGMENTATION, DNA-PROTEIN CROSS-LINKS, P-32 POSTLABELED NUCLEOTIDIC MODIFICATIONS, AND 8-HYDROXY-2'-DEOXYGUANOSINE IN THE LUNG BUT NOTIN THE LIVER OF RATS RECEIVING INTRATRACHEAL INSTILLATIONS OF CHROMIUM(VI) - CHEMOPREVENTION BY ORAL N-ACETYLCYSTEINE", Mutation research. Fundamental and molecular mechanisms of mutagenesis, 400(1-2), 1998, pp. 233-244

Abstract

An in vivo study was carried out with the objectives of evaluating (a) the localization of DNA lesions resulting from exposure to chromium(VI) by the respiratory route, (b) the molecular nature of DNA alterations, and (c) modulation of DNA damage by a known chemopreventive agent. To this purpose, Sprague-Dawley rats received intratracheal instillations of sodium dichromate (0.25 mg/kg body weight) for three consecutive days, and the day after the last treatment lung and liver were removed for DNA purification. The results showed a selective localizationof DNA lesions in the lung but not in the liver, which can be ascribed to toxicokinetics and metabolic characteristics of chromium(VI). DNAalterations included DNA-protein crosslinks, DNA fragmentation, nucleotidic modifications, and 8-hydroxy-2'-deoxyguanosine. The last two endpoints were evaluated, for the first time in chromium toxicology, by means of P-32 postlabeling procedures. This methodology was adapted tothe detection of the DNA damage produced by those reactive oxygen species which result from the intracellular reduction of chromium(Vr). The oral administration of the thiol N-acetylcysteine completely prevented any induction of DNA lesions in lung cells. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:26:02