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Titolo:
QUANTITATIVE STUDIES OF ENZYME-SUBSTRATE COMPARTMENTATION, FUNCTIONALCOUPLING AND METABOLIC CHANNELING IN MUSCLE-CELLS
Autore:
SAKS V; DOSSANTOS P; GELLERICH FN; DIOLEZ P;
Indirizzi:
UNIVERSITE JOSEPH FOURIER,LAB BIOENERGET,BP 53X F-38041 GRENOBLE FRANCE INST CHEM & BIOL PHYS TALLINN ESTONIA INSERM,U441 ATHEROSCLEROSE DETERMINISME CELLULAIRE & MOL BORDEAUX FRANCE MARTIN LUTHER UNIV HALLE WITTENBERG,MUSKEL LAB HALLE GERMANY UNIV BORDEAUX 2,CNRS,UMR 5536 BORDEAUX FRANCE
Titolo Testata:
Molecular and cellular biochemistry
fascicolo: 1-2, volume: 184, anno: 1998,
pagine: 291 - 307
SICI:
0300-8177(1998)184:1-2<291:QSOECF>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIAL CREATINE-KINASE; ATP TURNOVER RATES; IN-VIVO REGULATION; ENERGY-METABOLISM; CARDIAC MYOCYTES; CONFOCAL MICROSCOPY; SKELETAL-MUSCLES; OXIDATIVE-PHOSPHORYLATION; VENTRICULAR MYOCYTES; OXYGEN-CONSUMPTION;
Keywords:
CREATINE KINASE; MITOCHONDRIA; RESPIRATION; CONTRACTION; REGULATION; THERMODYNAMICS; COMPARTMENTATION; FUNCTIONAL COUPLING; METABOLIC CHANNELING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
91
Recensione:
Indirizzi per estratti:
Citazione:
V. Saks et al., "QUANTITATIVE STUDIES OF ENZYME-SUBSTRATE COMPARTMENTATION, FUNCTIONALCOUPLING AND METABOLIC CHANNELING IN MUSCLE-CELLS", Molecular and cellular biochemistry, 184(1-2), 1998, pp. 291-307

Abstract

Some historical aspects of development of the concepts of functional coupling, metabolic channelling, compartmentation and energy transfer networks are reviewed, Different quantitative approaches, including kinetic and mathematical modeling of energy metabolism, intracellular energy transfer and metabolic regulation of energy production and fluxesin the cells in vivo are analyzed. As an example of the system with metabolic channelling thermodynamic aspects of the functioning the mitochondrial creatine kinase functionally coupled to the oxidative phosphorylation are considered. The internal thermodynamics of the mitochondrial creatine kinase reaction is similar to that for other isoenzymes of creatine kinase, and the oxidative phosphorylation process specifically influences steps of association and dissociation of MgATP with the enzyme due to channelling of ATP from adenine nucleotide translocase. A new paradigm of muscle bioenergetics - the paradigm of energy transfer and feedback signaling networks based on analysis of compartmentation phenomena and structural and functional interactions in the cell is described. Analysis of the results of mathematical modeling of the compartmentalized energy transfer leads to conclusion that both calcium and ADP, which concentration changes synchronously in contraction cycle, may simultaneously activate oxidative phosphorylation in the muscle cells in vivo. The importance of the phosphocreatine circuit among other pathways of intracellular energy transfer network is discussed on the basis of the recent data published in the literature, with some experimental demonstration. The results of studies of perfused rat hearts with completely inhibited creatine kinase show significantly decreased work capacity and respectively, energy fluxes, in these hearts inspite of significant activation of adenylate kinase system (Dzeja ct al. this volume). These results, combined with those of mathematical analysis of the energy metabolism of hearts of transgenic mice with switched off creatine kinase isoenzymes confirm the importance of phosphocreatine pathway for energy transfer for cell function and energetics in mature heart and many other types of cells, as one of major parts of intracellular energy transfer network and metabolic regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:14:37