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Titolo:
INFLUENCE OF CD34 CELL SELECTION ON THE INCIDENCE OF MIXED CHIMERISM AND MINIMAL RESIDUAL DISEASE AFTER ALLOGENEIC UNRELATED DONOR TRANSPLANTATION
Autore:
SOCIE G; CAYUELA JM; RAYNAL B; ESPEROU H; FUND X; RAFFOUX C; DEVERGIE A; RIBAUD P; MAROLLEAU JP; PARQUET N; SIGAUX F; BRISON O; GLUCKMAN E;
Indirizzi:
HOP ST LOUIS,SERV HEMATOL GREFFE DE MOELLE,1 AVE CLAUDE VELLEFAUX F-75475 PARIS 10 FRANCE HOP ST LOUIS,UNITE RECH BIOL CELLULES SOUCHES F-75475 PARIS 10 FRANCE HOP ST LOUIS,INSERM,U462 PARIS FRANCE HOP ST LOUIS,ETABLISSEMENT TRANSFUS SANGUINE,LAB THERAPIE CELLULAIRE PARIS FRANCE HOP ST LOUIS,FRANCE GREFFE DE MOELLE PARIS FRANCE INST GUSTAVE ROUSSY,CNRS,URA 1967,LAB GENET ONCOL VILLEJUIF FRANCE
Titolo Testata:
Leukemia
fascicolo: 9, volume: 12, anno: 1998,
pagine: 1440 - 1446
SICI:
0887-6924(1998)12:9<1440:IOCCSO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW TRANSPLANTATION; POLYMERASE CHAIN-REACTION; CHRONIC MYELOID-LEUKEMIA; CHRONIC MYELOGENOUS LEUKEMIA; VERSUS-HOST DISEASE; FREE SURVIVAL; CHIMERISM; RELAPSE; RECIPIENT; PROPHYLAXIS;
Keywords:
CHIMERISM; RESIDUAL DISEASE; MARROW TRANSPLANTATION; UNRELATED DONOR; T CELL DEPLETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
G. Socie et al., "INFLUENCE OF CD34 CELL SELECTION ON THE INCIDENCE OF MIXED CHIMERISM AND MINIMAL RESIDUAL DISEASE AFTER ALLOGENEIC UNRELATED DONOR TRANSPLANTATION", Leukemia, 12(9), 1998, pp. 1440-1446

Abstract

Marrow transplantation from unrelated donors has been linked with an increased risk of graft-versus-host disease (GVHD). In an attempt to lower the risk of acute GVHD we used CD34 marrow cell selection for T cell depletion. Since T cell depletion has been linked to an increased risk of relapse and an increased risk of marrow failure, we used PCR amplification of minisatellite sequences to investigate donor cell engraftment and RT-PCR amplification of recurrent chromosomal translocations to investigate the residual disease post-transplant. Twenty-three patients who underwent BMT after positive selection of the CD34-positive cell population were studied. Results were then compared with those of 37 patients who underwent transplantation with unmanipulated marrowgraft. Among the 23 patients who received CD34(+) selected cell grafts, seven (30%) had evidence of full donor engraftment, 14 had evidenceof residual recipient cells (61%), one had a non-take, and one autologous bone marrow recovery. Analysis of the chimaerism status post-transplant in 36 patients who received unmanipulated marrow grafts showed that 31 patients (86%) had evidence of full donor engraftment. The difference in the incidence of mixed chimaerism profile between patients who received unmanipulated marrow graft and those receiving CD34(+) selected cell grafts was statistically significant (P<0.01). Nine patients who received CD34(+) selected cell grafts could be analysed for thepresence of minimal residual disease post-transplant (one with t(9;22) acute lymphoblastic leukaemia and eight with CML). In the patient transplanted for a Ph-positive acute leukaemia, and in two out of the eight patients with CML, the search fora fusion transcript was consistently negative after transplantation. Among the six patients with evidence of residual disease, three patients also had a mixed chimaerism profile and were given donor lymphocyte infusions. Minimal residual disease study was performed post-transplant in 16 patients who received unmanipulated marrow grafts. In 10 of 14 patients with CML, and in two patients with acute leukaemia the search for a fusion transcript was consistently negative after transplantation. The difference in the incidence of minimal residual disease between patients who received an unmanipulated marrow graft and those receiving CD34(+) selected cell graftswas not statistically significantly significant, but numbers of patients included in this analysis are still few. In conclusion, our study highlights the strong influence of graft manipulation on the incidenceof mixed chimaerism after transplantation from an unrelated donor.

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Documento generato il 07/07/20 alle ore 11:19:55