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Titolo:
SYNTHESIS OF GLUTAMINE, GLYCINE AND L0-FORMYL TETRAHYDROFOLATE IS COREGULATED WITH PURINE BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE
Autore:
DENIS V; DAIGNANFORNIER B;
Indirizzi:
INST BIOCHIM & GENET CELLULAIRES,1 RUE CAMILLE ST SAENS F-33077 BORDEAUX FRANCE INST BIOCHIM & GENET CELLULAIRES F-33077 BORDEAUX FRANCE
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 3, volume: 259, anno: 1998,
pagine: 246 - 255
SICI:
0026-8925(1998)259:3<246:SOGGAL>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTIONAL ACTIVATORS BAS1; MOLECULAR CHARACTERIZATION; NUCLEOTIDE-SEQUENCE; 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE; 3-PHOSPHOSERINE AMINOTRANSFERASE; HIS4 TRANSCRIPTION; ADE2 GENE; YEAST; CLONING; PROTEIN;
Keywords:
TRANSCRIPTION FACTORS BAS1P/BAS2P; GLN1/SHM2/MTD1; ADENINE REPRESSION; SACCHAROMYCES CEREVISIAE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
V. Denis e B. Daignanfornier, "SYNTHESIS OF GLUTAMINE, GLYCINE AND L0-FORMYL TETRAHYDROFOLATE IS COREGULATED WITH PURINE BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE", MGG. Molecular & general genetics, 259(3), 1998, pp. 246-255

Abstract

Glutamine, glycine and 10-formyl tetrahydrofolate are consumed duringde novo purine biosynthesis. We have found that, in Saccharomyces cerevisiae, synthesis of these cosubstrates is coregulated with synthesisof enzymes of the purine biosynthetic pathway. Analysis of three genes required for synthesis of glutamine, glycine and 10-formyl tetrahydrofolate (GLN1, SHM2 and MTD1, respectively) shows that their expression is repressed by adenine and requires the transcription factors Bas1pand Bas2p. Northern analysis reveals that regulation of SHM2 and MTD1expression by adenine takes place at the transcriptional level. We also show that Bas1p and Bas2p bind in vitro to the promoters of the SHM2 and MTD1 genes, and that mutations in the consensus Bas1p binding sequences strongly affect expression of these genes in vivo. Finally, wehave found that a SHM2-lacZ fusion is expressed at a significantly higher level in a bas2-2 disrupted strain than in bas1-2 or bas1-2 bas2-2 mutant strains. The BAS1-dependent, BAS2-independent expression of SHM2-lacZ suggests that, in the absence of Bas2p, Bas1p can interact with another protein partner to activate SHM2 expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 03:14:26