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Titolo:
INTEGRATING CYTOSOLIC CALCIUM SIGNALS INTO MITOCHONDRIAL METABOLIC RESPONSES
Autore:
ROBBGASPERS LD; BURNETT P; RUTTER GA; DENTON RM; RIZZUTO R; THOMAS AP;
Indirizzi:
UNIV MED & DENT NEW JERSEY,DEPT PHYSIOL & PHARMACOL NEWARK NJ 07103 UNIV MED & DENT NEW JERSEY,DEPT PHYSIOL & PHARMACOL NEWARK NJ 07103 UNIV BRISTOL,SCH MED SCI,DEPT BIOCHEM BRISTOL BS8 1TD AVON ENGLAND UNIV PADUA,DEPT BIOMED SCI I-35121 PADUA 17 ITALY UNIV PADUA,CNR,CTR STUDY BIOL MEMBRANES I-35121 PADUA 17 ITALY
Titolo Testata:
EMBO journal (Print)
fascicolo: 17, volume: 17, anno: 1998,
pagine: 4987 - 5000
SICI:
0261-4189(1998)17:17<4987:ICCSIM>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFUSED-RAT-LIVER; PYRUVATE-DEHYDROGENASE ACTIVITY; ISOLATED HEPATOCYTES; 2-OXOGLUTARATE DEHYDROGENASE; MAMMALIAN MITOCHONDRIA; CA2+ CONCENTRATION; MATRIX VOLUME; INTACT-CELLS; ACTIVATION; GLUCAGON;
Keywords:
CALCIUM; HEPATOCYTE; MITOCHONDRIA; PROTON MOTIVE FORCE; PYRUVATE DEHYDROGENASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
L.D. Robbgaspers et al., "INTEGRATING CYTOSOLIC CALCIUM SIGNALS INTO MITOCHONDRIAL METABOLIC RESPONSES", EMBO journal (Print), 17(17), 1998, pp. 4987-5000

Abstract

Stimulation of hepatocytes with vasopressin evokes increases in cytosolic free Ca2+ ([Ca2+](c)) that are relayed into the mitochondria, where the resulting mitochondrial Ca2+ ([Ca2+](m)) increase regulates intramitochondrial Ca2+-sensitive targets. To understand how mitochondriaintegrate the [Ca2+](c) signals into a final metabolic response, we stimulated hepatocytes with high vasopressin doses that generate a sustained increase in [Ca2+](c). This elicited a synchronous, single spikeof [Ca2+](m) and consequent NAD(P)H formation, which could be relatedto changes in the activity state of pyruvate dehydrogenase (PDH) measured in parallel. The vasopressin-induced [Ca2+](m) spike evoked a transient increase in NAD(P)H that persisted longer than the [Ca2+](m) increase. In contrast, PDH activity increased biphasically, with an initial rapid phase accompanying the rise in [Ca2+](m), followed by a sustained secondary activation phase associated with a decline in cellularATP. The decline of NAD(P)H in the face of elevated PDH activity occurred as a result of respiratory chain activation, which was also manifest in a calcium-dependent increase in the membrane potential and pH gradient components of the proton motive force (PMF). This is the firstdirect demonstration that Ca2+-mobilizing hormones increase the PMF in intact cells, Thus, Ca2+ plays an important role in signal transduction from cytosol to mitochondria, with a single [Ca2+](m) spike evoking a complex series of changes to activate mitochondrial oxidative metabolism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:08:37