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Titolo:
PRELIMINARY-REPORT ON THE DEVELOPMENT AND CHARACTERIZATION OF RABBIT CLITORAL SMOOTH-MUSCLE CELL-CULTURE
Autore:
SADEGHINEJAD H; MORELAND RB; TRAISH AM; NEHRA A; AZADZOI KM; GOLDSTEIN I;
Indirizzi:
BOSTON UNIV,SCH MED,DEPT UROL,720 HARRISON AVE BOSTON MA 02118 BOSTON UNIV,SCH MED,DEPT UROL BOSTON MA 02118 UNIV MED & DENT NEW JERSEY,UMD NEWARK DE 00000
Titolo Testata:
International journal of impotence research
fascicolo: 3, volume: 10, anno: 1998,
pagine: 165 - 169
SICI:
0955-9930(1998)10:3<165:POTDAC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMING GROWTH-FACTOR; CORPUS CAVERNOSUM; RECEPTORS; MEN;
Keywords:
FEMALE SEXUAL DYSFUNCTION; SMOOTH MUSCLE CELL CULTURE; CLITORIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
H. Sadeghinejad et al., "PRELIMINARY-REPORT ON THE DEVELOPMENT AND CHARACTERIZATION OF RABBIT CLITORAL SMOOTH-MUSCLE CELL-CULTURE", International journal of impotence research, 10(3), 1998, pp. 165-169

Abstract

Purpose: Scientific model systems for physiological evaluation and investigation of pathophysiologies in clitoral function have been limited. The aim was to develop a New Zealand White rabbit clitoral corpus cavernosum smooth muscle cell culture. Methods: Clitoral corpus cavernosum erectile tissue was harvested and placed in culture. Clitoral smooth muscle cells which migrated out from explants were grown to confluence and subcultured. Characterizations were performed by morphologicaland biochemical analyses. Results: The cells exhibited typical morphologic characteristics of smooth muscle cells. Indirect immunofluorescence studies confirmed the presence of a-smooth muscle cell actin. Androgen and estrogen receptors were detected by specific antibodies and binding studies. The cells expressed subtypes of TGF-beta receptors. Treatment with 80 pM TGF-beta(1) 24 h resulted in induction and/or increased availability of TGF-beta receptors. Conclusions: An in-vitro cellculture system using rabbit clitoral smooth muscle cells was developed. These smooth muscle cells retain their biochemical and functional integrity. This in-vitro cell culture system may facilitate studies aimed at understanding the molecular basis of female sexual function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:23:32