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Titolo:
LEAD-INDUCED CHANGES IN SPERMATOZOA FUNCTION AND METABOLISM
Autore:
HSU PC; HSU CC; LIU MY; CHEN LY; GUO YL;
Indirizzi:
NATL CHENG KUNG UNIV,COLL MED,DEPT ENVIRONM & OCCUPAT HLTH,61 SHIAO TONG RD TAINAN 70428 TAIWAN NATL CHENG KUNG UNIV,COLL MED,DEPT ENVIRONM & OCCUPAT HLTH TAINAN 70428 TAIWAN CHIA NAN COLL PHARM & SCI,DEPT IND SAFETY & HYG TAINAN TAIWAN NATL CHENG KUNG UNIV,COLL MED,DEPT OBSTET & GYNECOL TAINAN 70101 TAIWAN NATL CHENG KUNG UNIV,COLL MED,DEPT PHYSIOL TAINAN 70101 TAIWAN
Titolo Testata:
Journal of toxicology and environmental health. Part A
fascicolo: 1, volume: 55, anno: 1998,
pagine: 45 - 64
SICI:
1528-7394(1998)55:1<45:LCISFA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM ACROSOME REACTION; HYDROGEN-PEROXIDE; CAPACITATION INVITRO; ZONA PELLUCIDA; ION INFLUX; RAT; MOUSE; EXPOSURE; TESTOSTERONE; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
P.C. Hsu et al., "LEAD-INDUCED CHANGES IN SPERMATOZOA FUNCTION AND METABOLISM", Journal of toxicology and environmental health. Part A, 55(1), 1998, pp. 45-64

Abstract

The relationships between sperm reactive oxygen species (ROS) generation, the capacitation process and acrosome reaction, and the sperm-oocyte penetration late (SOPR) were investigated to understand the effectof lead toxicity on sperm functions and the mechanisms of these effects. Male Sprague-Dawley rats received weekly intraperitoneal injections of 20 mg or 50 mg lead acetate/kg or 20 mg or 50 mg sodium acetate/kg (control) for 6 wk. Serum testosterone was measured by radioimmunoassay. In cauda epididymal spermatozoa, the chemiluminescence was measured to evaluate the sperm ROS generation. Chlortetracycline fluorescence assay was used to study the status of capacitation and acrosome reaction on fresh cauda epididymal spermatozoa and after 2, 4, or 24 h of incubation with 5 mg/ml bovine serum albumin. In lead-exposed rats, the serum testosterone levels were reduced, and the percentage of capacitation and the chemiluminescence were significantly increased in freshcauda epididymal spermatozoa. The serum testosterone levels were negatively associated with the percentage of acrosome-reacted spermatozoa. Sperm chemiluminescence was positively correlated with the percentageof both capacitated and acrosome-reacted spermatozoa. The SOPR was negatively associated with the percentage of both capacitated and acrosome-reacted spermatozoa. In summary, this study showed that male rats exposed to lead had decreased serum testosterone levels and that this metal produced early onset of capacitation by one of the pathways of ROS generation. These effects might consequently result in premature acrosome reaction and reduced zona-intact oocyte-penetrating capability.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:31:34