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Titolo:
EFFICIENT DISPLAY OF AN HCV CDNA EXPRESSION LIBRARY AS C-TERMINAL FUSION TO THE CAPSID PROTEIN-D OF BACTERIOPHAGE-LAMBDA
Autore:
SANTINI C; BRENNAN D; MENNUNI C; HOESS RH; NICOSIA A; CORTESE R; LUZZAGO A;
Indirizzi:
IST RIC BIOL MOL P ANGELETTI ROME ITALY IST RIC BIOL MOL P ANGELETTI ROME ITALY DUPONT MERCK PHARMACEUT CO,EXPT STN WILMINGTON DE 19880
Titolo Testata:
Journal of Molecular Biology
fascicolo: 1, volume: 282, anno: 1998,
pagine: 125 - 135
SICI:
0022-2836(1998)282:1<125:EDOAHC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MONOCLONAL-ANTIBODY; RANDOM PEPTIDE LIBRARIES; B-CELL EPITOPE; PHAGE DISPLAY; SURFACE DISPLAY; FILAMENTOUS BACTERIOPHAGE; HUMAN SERA; VIRUS; SELECTION; VECTOR;
Keywords:
LAMBDA; D PROTEIN; PHAGE DISPLAY; CDNA LIBRARY; MONOCLONAL ANTIBODIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
C. Santini et al., "EFFICIENT DISPLAY OF AN HCV CDNA EXPRESSION LIBRARY AS C-TERMINAL FUSION TO THE CAPSID PROTEIN-D OF BACTERIOPHAGE-LAMBDA", Journal of Molecular Biology, 282(1), 1998, pp. 125-135

Abstract

We describe the construction and characterization of a hepatitis C virus (HCV) cDNA expression library displayed as a fusion to the carboxyterminus of the capsid protein D of bacteriophage lambda, cDNA inserts were obtained by tagged random-priming of the HCV genome and cloned into a lambda vector from which chimeric phage bearing both wild-type D protein and D fusion products on the capsid surface were produced. The resulting library was affinity-selected with anti-HCV human monoclonal antibodies recognizing linear or conformational epitopes, and human sera from HCV-infected patients. Selection was monitored by immune-screening experiments, ELISA, and sequence analysis of positive clones. The performance of this Library was compared with two additional HCV cDNA display libraries generated as N-terminal fusions to the III and WI capsid proteins of filamentous phage M13. The results obtained demonstrate the great potential of the lambda display system for constructing complex cDNA libraries for natural ligand discovery. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 12:51:58