Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MEDIUM CHANGE AMPLIFIES MITOGEN-ACTIVATED PROTEIN KINASE-MEDIATED PROSTAGLANDIN E-2 SYNTHESIS IN SWISS 3T3 FIBROBLASTS
Autore:
NAKATANI K; NAKAHATA N; HAMADA Y; TSURUFUJI S; OHIZUMI Y;
Indirizzi:
TOHOKU UNIV,FAC PHARMACEUT SCI,DEPT PHARMACEUT MOL BIOL,AOBA KU SENDAI MIYAGI 9808578 JAPAN TOHOKU UNIV,FAC PHARMACEUT SCI,DEPT PHARMACEUT MOL BIOL,AOBA KU SENDAI MIYAGI 9808578 JAPAN INST CYTOSIGNAL RES INC,SHINAGAWA KU TOKYO 1400005 JAPAN
Titolo Testata:
European journal of pharmacology
fascicolo: 1, volume: 356, anno: 1998,
pagine: 91 - 100
SICI:
0014-2999(1998)356:1<91:MCAMPK>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOSOLIC PHOSPHOLIPASE A(2); HUMAN ASTROCYTOMA-CELLS; TYROSINE PHOSPHORYLATION; ARACHIDONIC-ACID; GROWTH-FACTOR; MESSENGER-RNA; MAP KINASE; EXPRESSION; MASTOPARAN; GENERATION;
Keywords:
MEDIUM CHANGE; BRADYKININ; PROSTAGLANDIN E-2; PHOSPHOLIPASE A(2); TYROSINE KINASE; MITOGEN-ACTIVATED PROTEIN KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
K. Nakatani et al., "MEDIUM CHANGE AMPLIFIES MITOGEN-ACTIVATED PROTEIN KINASE-MEDIATED PROSTAGLANDIN E-2 SYNTHESIS IN SWISS 3T3 FIBROBLASTS", European journal of pharmacology, 356(1), 1998, pp. 91-100

Abstract

In Swiss 3T3 fibroblasts, changing the culture medium prior to stimulation resulted in an augmentation of bradykinin-induced prostaglandin E-2 synthesis. The augmentation depended on the duration of the exposure to the fresh medium, with a maximum effect at 1 h. Fetal calf serumin the fresh medium was essential for augmented prostaglandin E-2 synthesis. The medium change slightly augmented the bradykinin-induced increase in intracellular free Ca'+ concentration and phosphoinositide hydrolysis with a different time course from that for prostaglandin E-2synthesis. 4',5,7-Trihydroxyisoflavone (genistein) and 3,4-dihydroxybenzylidene-malononitrile (tyrphostin 23), inhibitors of tyrosine kinases, and 2'-amino-3'-methoxyflavone (PD98059), an inhibitor of mitogen-activated protein kinase (MAPK) kinase, attenuated the increase in prostaglandin E-2 synthesis. Bradykinin caused phosphorylation of cytosolic phospholipase A(2) and p42/p44 MAPK, which was augmented by the medium change. From the results, it is concluded that activation of MAPK and cycosolic phospholipase A(2) is involved in the augmentation of prostaglandin E-2 synthesis produced by the medium change. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 09:06:09