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Titolo:
ISOLATION AND CHARACTERIZATION OF ANTIBODIES WHICH SPECIFICALLY RECOGNIZE THE PEPTIDE ENCODED BY EXON-7 (V2) OF THE HUMAN CD44 GENE
Autore:
BORGYA A; WOODMAN A; SUGIYAMA M; DONIE F; KOPETZKI E; MATSUMURA Y; TARIN D;
Indirizzi:
UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT PATHOL & BACTERIOL OXFORD OX3 9DU ENGLAND UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT PATHOL & BACTERIOL OXFORD OX3 9DU ENGLAND BOEHRINGER MANNHEIM GMBH D-82372 PENZBERG GERMANY
Titolo Testata:
JCP. Clinical molecular pathology
fascicolo: 5, volume: 48, anno: 1995,
pagine: 241 - 250
SICI:
1355-2910(1995)48:5<241:IACOAW>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH ENDOTHELIUM; LYMPH-NODE; RECEPTOR; GLYCOPROTEIN; EXPRESSION; CELLS; HYALURONATE; CARCINOMAS; MOLECULE; PROTEINS;
Keywords:
CD44 ANTIBODIES; TUMOR MARKER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
A. Borgya et al., "ISOLATION AND CHARACTERIZATION OF ANTIBODIES WHICH SPECIFICALLY RECOGNIZE THE PEPTIDE ENCODED BY EXON-7 (V2) OF THE HUMAN CD44 GENE", JCP. Clinical molecular pathology, 48(5), 1995, pp. 241-250

Abstract

Aims-Exon 7 of the human CD44 gene is overexpressed in many commonly occurring carcinomas. The aim of the study was to explore the diagnostic and therapeutic potential of this frequent abnormality. Methods-A new monoclonal antibody (mAb, M-23.6.1) and a polyclonal antibody (pAb,S-6127) to the corresponding antigen were raised by immunising mice and sheep, respectively, with a specially constructed fusion protein HIV2 (gp32)-CD44 exon 7. Results-Characterisation of mAb, M-23.6.1 by ELISA, western blotting, immunocytochemistry, and FAGS analysis confirmedthat it specifically recognises an epitope in the region between amino acids 19 and 33 of the peptide encoded by this exon. Western blotting experiments with two cell lines, RT112 and ZR75-1, known from RT-PCRdata to be over-transcribing the exon, yielded a monospecific band ofapproximately 220 kDa, and immunocytochemistry showed discrete membrane staining on the same cell lines. Fluorescent antibody cell sorting (FAGS) revealed binding to greater than 90% of the cells of each of these lines. Specificity of recognition of the antigen was shown by inhibition of the precise immunoreactivity typically seen in ELISA and Western blots, by pre-incubation with synthetic exon 7 peptide or fragments of it. Conclusions-The new antibodies will be useful tools for the further analysis of abnormal CD44 isoforms and their clinical implications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 03:57:35