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Titolo:
CONSTITUTIVE IL-10 PRODUCTION ACCOUNTS FOR THE HIGH NK SENSITIVITY, LOW MHC CLASS-I EXPRESSION, AND POOR TRANSPORTER ASSOCIATED WITH ANTIGEN-PROCESSING (TAP)-1 2 FUNCTION IN THE PROTOTYPE NK TARGET YAC-1/
Autore:
PETERSSON M; CHARO J; SALAZARONFRAY F; NOFFZ G; MOHAUPT M; QIN ZH; KLEIN G; BLANKENSTEIN T; KIESSLING R;
Indirizzi:
KAROLINSKA INST,MTC S-17177 STOCKHOLM SWEDEN MAX DELBRUCK CTR MOL MED BERLIN GERMANY KAROLINSKA HOSP,RADIUMHEMMET,DEPT EXPT ONCOL S-10401 STOCKHOLM SWEDEN
Titolo Testata:
The Journal of immunology (1950)
fascicolo: 5, volume: 161, anno: 1998,
pagine: 2099 - 2105
SICI:
0022-1767(1998)161:5<2099:CIPAFT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER CELLS; CYTOTOXIC T-CELLS; DOWN-REGULATION; ONCOGENIC ADENOVIRUS-12; SELECTIVE EXPRESSION; ENCODED TRANSPORTER; INTERFERON-GAMMA; MOUSE LYMPHOMA; MELANOMA-CELLS; MESSENGER-RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
M. Petersson et al., "CONSTITUTIVE IL-10 PRODUCTION ACCOUNTS FOR THE HIGH NK SENSITIVITY, LOW MHC CLASS-I EXPRESSION, AND POOR TRANSPORTER ASSOCIATED WITH ANTIGEN-PROCESSING (TAP)-1 2 FUNCTION IN THE PROTOTYPE NK TARGET YAC-1/", The Journal of immunology (1950), 161(5), 1998, pp. 2099-2105

Abstract

Tumor cells that are treated with rIL-10 or transfected with the IL-10 gene Show phenotypic changes. These include low but peptide-inducible expression of MHC class I, low sensitivity to specific CTL-mediated lysis, and increased NK sensitivity. In vitro-established mouse tumor lines were screened for IL-10 expression and production, and a large proportion of plasmocytomas or T cell lymphomas were found to produce IL-10, Since one of these lines was the prototype NK target cell YAC-1,we investigated whether the high IL-10 production of this cell line was related to its high NK sensitivity and its defects in MHC class I expression. The decrease in H-2 expression following the in vitro culture of in vivo-passaged YAC-1 cells was accompanied by a gradual increase in IL-10 production, whereas the reverse was found when passing in vitro-grown YAC-1 in vivo as an ascites tumor in syngenic mice. In addition, differences in YAC-1 MHC class I expression correlated with alterations in the functional activity of TAP-1/2 proteins. YAC-1 cells that were transduced with a retroviral IL-10 antisense construct (Y-IL-10 AS) only produced about half of the IL-10 that was produced by YAC-I transduced with the control construct (Y-IL-10 Mock). Relative to Y-IL-10 Mock cells, the expression of H-2 on Y-IL-10 AS cells was markedly increased, and Mt sensitivity was decreased. These data argue for amechanism wherein IL-10 production is causally related to the low H-2expression, decreased TAP function, and high NK sensitivity of YAC-1 cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 21:27:17