Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
TRANSFORMATION OF 2 POTATO CULTIVARS SPUNTA AND CLAUSTAR (SOLANUM-TUBEROSUM) WITH LETTUCE MOSAIC-VIRUS COAT PROTEIN GENE AND HETEROLOGOUS IMMUNITY TO POTATO-VIRUS-Y
Autore:
HASSAIRI A; MASMOUDI K; ALBOUY J; ROBAGLIA C; JULLIEN M; ELLOUZ R;
Indirizzi:
CTR BIOTECHNOL SFAX,LAB BIOTECHNOL VEGETALE,BP 358-3018 SFAX TUNISIA INRA,PATHOL VEGETALE STN F-78026 VERSAILLES FRANCE INRA,BIOL CELLULAIRE LAB F-78026 VERSAILLES FRANCE
Titolo Testata:
PLANT SCIENCE
fascicolo: 1, volume: 136, anno: 1998,
pagine: 31 - 42
SICI:
0168-9452(1998)136:1<31:TO2PCS>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGEN-DERIVED RESISTANCE; AGROBACTERIUM-MEDIATED TRANSFORMATION; TRANSGENIC RUSSET BURBANK; TOBACCO ETCH POTYVIRUS; NUCLEOTIDE-SEQUENCE; PLANTS; INFECTION; PROTECTION; RNA; EXPRESSION;
Keywords:
HETEROLOGOUS-RESISTANCE; TRANSGENIC POTATO; LMV; PVY; VIRUS RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
A. Hassairi et al., "TRANSFORMATION OF 2 POTATO CULTIVARS SPUNTA AND CLAUSTAR (SOLANUM-TUBEROSUM) WITH LETTUCE MOSAIC-VIRUS COAT PROTEIN GENE AND HETEROLOGOUS IMMUNITY TO POTATO-VIRUS-Y", PLANT SCI, 136(1), 1998, pp. 31-42

Abstract

The coat protein (CP) gene of the lettuce mosaic potyvirus (LMV) was introduced into two potato cultivars-Spunta and Claustar-via Agrobacterium tumefaciens-mediated transformation. Gene expression was verifiedby Southern, Northern and Western blot. The regenerated plants have been tested for resistance against potato virus Y (PVY-0) in greenhouse. We observed an immune phenotype in four transgenic lines expressing the CP-LMV protein at different delectable levels. However, in two other transgenic plant lines, symptoms were developed at a frequency of 25 and 60% with a delay in their appearance. Transgene transcripts of the CP-LMV gene were detected by Northern blot in these two lines, but the protein could not be detected by Western blot. The correlation between the presence of the CP protein at a detectable level and the expression of an immune phenotype is discussed. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 12:34:06