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Titolo:
IN-VITRO GENOTYPIC VARIATION OF CAMPYLOBACTER-COLI DOCUMENTED BY PULSED-FIELD GEL-ELECTROPHORETIC DNA PROFILING, IMPLICATIONS FOR EPIDEMIOLOGIC STUDIES
Autore:
ON SLW;
Indirizzi:
DANISH VET LAB,BULOWSVEJ 27 DK-1790 COPENHAGEN V DENMARK
Titolo Testata:
FEMS microbiology letters
fascicolo: 2, volume: 165, anno: 1998,
pagine: 341 - 346
SICI:
0378-1097(1998)165:2<341:IGVOCD>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
JEJUNI; IDENTIFICATION; HELICOBACTER; GENETICS; SCHEME;
Keywords:
CAMPYLOBACTER COLI; PFGE; GENOTYPIC VARIATION; NUMERICAL ANALYSIS; EVOLUTIONARY GENETICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
S.L.W. On, "IN-VITRO GENOTYPIC VARIATION OF CAMPYLOBACTER-COLI DOCUMENTED BY PULSED-FIELD GEL-ELECTROPHORETIC DNA PROFILING, IMPLICATIONS FOR EPIDEMIOLOGIC STUDIES", FEMS microbiology letters, 165(2), 1998, pp. 341-346

Abstract

Six isolates of Campylobacter coli from different pig herds were subcultured up to 50 times over a 6-month period and DNA samples suitable for pulsed-field gel electrophoretic (PFGE) profiling prepared at regular (1, 20, 40 and 50 passages) intervals. In 5/6 strains, changes in the banding patterns of Sma1, Sal1 and/or BamH1 digests were observed. In one such strain the differences were considered artifactual. However, significant alterations in PFGE profiles between subcultures of four strains were seen, irrespective of the restriction enzyme used. Spontaneous intramolecular genomic rearrangements were considered the most likely mechanism for the changes observed. A numerical analysis based upon the combined distribution of Sma1- and Sal1-derived fragments clustered most strain subcultures together, with the exception of thosefrom one isolate which were divided into two clusters. The effect of spontaneous genetic change on PFGE profiles must be considered when evaluating strain relationships. Numerical techniques may aid data interpretation but results must be evaluated cautiously. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier ScienceB.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 21:02:29