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Titolo:
PACLITAXEL-INDUCED APOPTOSIS IS ASSOCIATED WITH EXPRESSION AND ACTIVATION OF C-MOS GENE-PRODUCT IN HUMAN OVARIAN-CARCINOMA SKOV3 CELLS
Autore:
LING YH; YANG YD; TORNOS C; SINGH BR; PEREZSOLER R;
Indirizzi:
UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT THORAC HEAD & NECK MED ONCOL,SECT EXPT THERAPY,BOX 080 HOUSTON TX 77030 UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT PATHOL HOUSTON TX 77030 UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT MOL PATHOL HOUSTON TX 77030
Titolo Testata:
Cancer research
fascicolo: 16, volume: 58, anno: 1998,
pagine: 3633 - 3640
SICI:
0008-5472(1998)58:16<3633:PAIAWE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
METAPHASE-II ARREST; PROTEIN-KINASE; ONCOGENE PRODUCT; SOMATIC-CELLS; NIH3T3 CELLS; MAP KINASE; CYCLIN-B; OOCYTES; TAXOL; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
Y.H. Ling et al., "PACLITAXEL-INDUCED APOPTOSIS IS ASSOCIATED WITH EXPRESSION AND ACTIVATION OF C-MOS GENE-PRODUCT IN HUMAN OVARIAN-CARCINOMA SKOV3 CELLS", Cancer research, 58(16), 1998, pp. 3633-3640

Abstract

The c-Mos gene product is a component of the cytostatic factor and, as such, stabilizes the maturation-promoting factor causing cell-cycle blockade at metaphase II in unfertilized eggs. The potential role of c-Mos in regulating cell-cycle progression and cell death in somatic cells remains unknown. We studied whether paclitaxel-induced M-phase arrest and apoptosis are associated with c-Mos gene expression and activation in SKOV3 ovarian carcinoma cells. The first cellular effect observed with continuous exposure to 50 ng/ml paclitaxel (ID50) was mitoticarrest with an increase in the accumulation of cyclin B1 and stimulation of cdc2/cyclin B1 kinase in a time-dependent manner during a 36-h incubation. DNA fragmentation determined by agarose gel electrophoresis and quantitation of [H-3]thymidine-prelabeled genomic DNA was a later event, first detected at 24 h and peaking at 48 h (later time pointswere not studied). Induction of the c-Mos gene expression and activation were determined by Western blot analysis, immunoprecipitation using a polyclonal anti-mos antibody, reverse transcription-PCR assay, andP-32-ATP incorporation into c-Mos protein or the substrate of glutathione S-transferase mitogen-activated protein kinase kinase, respectively. Both induction and activation were clearly detected after 24 h of exposure to paclitaxel concentrations of >50 ng/ml, coinciding with drug-induced apoptosis, Mitogen-activated protein kinase activation preceded c-Mos gene induction. Paclitaxel-induced c-Mos gene expression was completely abrogated by cycloheximide and actinomycin D. Mos gene expression was also induced in SKOV3 cells that were treated with vinblastine but not in those that were treated with camptothecin, etoposide,or cisplatin. We concluded that tubulin-disturbing agents induce c-Mos gene expression and activation in SKOV3 ovarian carcinoma cells and that such an effect occurs after mitotic blockade and coincides with drug-induced apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 09:29:01