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Titolo:
TOLYPORPHIN - A NATURAL PRODUCT FROM CYANOBACTERIA WITH POTENT PHOTOSENSITIZING ACTIVITY AGAINST TUMOR-CELLS IN-VITRO AND IN-VIVO
Autore:
MORLIERE P; MAZIERE JC; SANTUS R; SMITH CD; PRINSEP MR; STOBBE CC; FENNING MC; GOLBERG JL; CHAPMAN JD;
Indirizzi:
MUSEUM NATL HIST NAT,INSERM,U312,LAB PHOTOBIOL F-75231 PARIS 05 FRANCE MUSEUM NATL HIST NAT,INSERM,U312,LAB PHOTOBIOL F-75231 PARIS 05 FRANCE UNIV PICARDIE,HOP NORD,BIOCHIM LAB F-80054 AMIENS 01 FRANCE FOX CHASE CANC CTR,DEPT PHARMACOL PHILADELPHIA PA 19111 FOX CHASE CANC CTR,DEPT RADIAT ONCOL PHILADELPHIA PA 19111 UNIV WAIKATO,DEPT CHEM HAMILTON NEW ZEALAND
Titolo Testata:
Cancer research
fascicolo: 16, volume: 58, anno: 1998,
pagine: 3571 - 3578
SICI:
0008-5472(1998)58:16<3571:T-ANPF>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED HUMAN-FIBROBLASTS; PHOTODYNAMIC THERAPY; PHOTOFRIN-II; DERIVATIVES; PORPHYRINS; INVITRO; PHOTOTOXICITY; RESISTANCE; OXYGEN; ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
P. Morliere et al., "TOLYPORPHIN - A NATURAL PRODUCT FROM CYANOBACTERIA WITH POTENT PHOTOSENSITIZING ACTIVITY AGAINST TUMOR-CELLS IN-VITRO AND IN-VIVO", Cancer research, 58(16), 1998, pp. 3571-3578

Abstract

Tolyporphin (TP), a porphyrin extracted from cyanobacteria, was foundto be a very potent photosensitizer of EMT-6 tumor cells grown both iii vitro as suspensions or monolayers and in vivo in tumors implanted on the backs of C.B17/Icr severe combined immunodeficient mice. Thus, during photodynamic treatment (PDT) of EMT-6 tumor cells in vitro, thephotokilling effectiveness of TP measured as the product of the reciprocal of D-50 (the light dose necessary to kill 50% of cells) and the concentration of TP is similar to 5000 times higher than that of Photofrin II (PII), the only PDT photosensitizer thus far approved for clinical trials. TP almost exclusively localizes in the perinuclear regionand specifically in the endoplasmic reticulum (ER), as shown by microspectrofluorometry on single living EMT-6 cells costained with the ER and/or Golgi fluorescent vital probes, 3,3'-dihexyloxacarbocyanine iodide and thyl-BODIPY(R))-1-pentanoyl]-D-erythro-sphingosine (Molecular Probes, Eugene, OR). As a result, the singlet oxygen-mediated photodynamic activity of TP induces an effective inactivation of the acyl CoA:cholesterol-O-acyltransferase, a sensitive marker of ER membrane integrity and alterations of the nuclear membrane. In vivo, with the EMT-6 mouse tumor model, an exceptional effectiveness is also observed as compared to that of PII and other second generation photosensitizers of the pheophorbide class, which are themselves much more potent than PII, The outstanding PDT activity of TP observed ill vivo may be due to its unique biodistribution properties, in particular much less extraction by the liver, resulting in a higher delivery to other tissues, including tumor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 14:16:44