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Titolo:
CARRIER-MEDIATED RELEASE, TRANSPORT RATES, AND CHARGE-TRANSFER INDUCED BY AMPHETAMINE, TYRAMINE, AND DOPAMINE IN MAMMALIAN-CELLS TRANSFECTED WITH THE HUMAN DOPAMINE TRANSPORTER
Autore:
SITTE HH; HUCK S; REITHER H; BOEHM S; SINGER EA; PIFL C;
Indirizzi:
UNIV VIENNA,INST BIOCHEM PHARMACOL,BORSCHKEGASSE 8A A-1090 VIENNA AUSTRIA UNIV VIENNA,INST BIOCHEM PHARMACOL A-1090 VIENNA AUSTRIA UNIV VIENNA,INST NEUROPHARMACOL A-1090 VIENNA AUSTRIA UNIV VIENNA,INST PHARMACOL A-1090 VIENNA AUSTRIA
Titolo Testata:
Journal of neurochemistry
fascicolo: 3, volume: 71, anno: 1998,
pagine: 1289 - 1297
SICI:
0022-3042(1998)71:3<1289:CRTRAC>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORPUS STRIATUM; CATECHOLAMINE TRANSPORTERS; INDUCED CURRENTS; COCAINE; SODIUM; NOREPINEPHRINE; EFFLUX; NORADRENALINE; SYNAPTOSOMES; EXPRESSION;
Keywords:
HEK 293 CELLS; HUMAN DOPAMINE TRANSPORTER; AMPHETAMINE; UPTAKE; PATCH CLAMP; RELEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
H.H. Sitte et al., "CARRIER-MEDIATED RELEASE, TRANSPORT RATES, AND CHARGE-TRANSFER INDUCED BY AMPHETAMINE, TYRAMINE, AND DOPAMINE IN MAMMALIAN-CELLS TRANSFECTED WITH THE HUMAN DOPAMINE TRANSPORTER", Journal of neurochemistry, 71(3), 1998, pp. 1289-1297

Abstract

Amphetamine and related substances induce dopamine release. Accordingto a traditional explanation, this dopamine release occurs in exchange for amphetamine by means of the dopamine transporter (DAT). We tested this hypothesis in human embryonic kidney 293 cells stably transfected with the human DAT by measuring the uptake of dopamine, tyramine, and D- and L-amphetamine as well as substrate-induced release of preloaded N-methyl-4-[H-3]phenylpyridinium ([H-3]MPP+). The uptake of substrates was sodium-dependent and was inhibited by ouabain and cocaine, which also prevented substrate-induced release of MPP+. Patch-clamp recordings revealed that all four substrates elicited voltage-dependent inward currents (on top of constitutive leak currents) that were prevented by cocaine. Whereas individual substrates had similar affinities inrelease, uptake, and patch-clamp experiments, maximal effects displayed remarkable differences. Hence, maximal effects in release and current induction were similar to 25% higher for D-amphetamine as compared with the other substrates. By contrast, dopamine was the most efficacious substrate in uptake experiments, with its maximal initial uptake rate exceeding those of amphetamine and tyramine by factors of 20 and 4, respectively. Our experiments indicate a poor correlation between substrate-induced release and the transport of substrates, whereas the ability of substrates to induce currents correlates well with their releasing action.

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Documento generato il 04/04/20 alle ore 12:25:26