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Titolo:
ACTIVATION OF RECEPTOR GENE-TRANSCRIPTION IS REQUIRED TO MAINTAIN CELL SENSITIZATION AFTER AGONIST EXPOSURE - STUDY ON NEUROTENSIN RECEPTOR
Autore:
NAJIMI M; SOUAZE F; MENDEZ M; HERMANS E; BERBAR T; ROSTENE W; FORGEZ P;
Indirizzi:
HOP ST ANTOINE,INSERM U339,184 RUE FAUBOURG ST ANTOINE F-75012 PARIS FRANCE HOP ST ANTOINE,INSERM U339 F-75012 PARIS FRANCE UNIV CATHOLIQUE LOUVAIN,PHARMACOL LAB B-1200 BRUSSELS BELGIUM INST MEXICANO PSIQUIATRIA MEXICO CITY 14370 DF MEXICO
Titolo Testata:
The Journal of biological chemistry
fascicolo: 34, volume: 273, anno: 1998,
pagine: 21634 - 21641
SICI:
0021-9258(1998)273:34<21634:AORGIR>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE-HYDROXYLASE GENE; BOND PSEUDOPEPTIDE ANALOGS; MESSENGER-RNA LEVELS; SMOOTH-MUSCLE CELLS; QUANTITATIVE RT-PCR; CALCIUM MOBILIZATION; DOWN-REGULATION; G-PROTEIN; DIFFERENTIAL REGULATION; RAPID DESENSITIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
M. Najimi et al., "ACTIVATION OF RECEPTOR GENE-TRANSCRIPTION IS REQUIRED TO MAINTAIN CELL SENSITIZATION AFTER AGONIST EXPOSURE - STUDY ON NEUROTENSIN RECEPTOR", The Journal of biological chemistry, 273(34), 1998, pp. 21634-21641

Abstract

Neurotensin (NT) acts through specific G protein-coupled receptors toinduce effects in the central nervous system and periphery. In this study we have shown that in the human neuroblastoma cell Line CHP 212, an NT agonist, JMV 449, induced high affinity neurotensin receptor (NTR) gene activation. I-125-NT binding of cells challenged with JMV 449 rapidly decreased then reappeared and subsequently stabilized at 50% of the control values after 48 h of agonist exposure. These receptors, which reappeared at the cell surface, are as active as those found in control cells as demonstrated by Ca2+ mobilization. Furthermore, the tyrosine hydroxylase (TH) gene, a known NT target gene, remained activated after prolonged NT agonist exposure in this cell line. In the murine neuroblastoma cell line, N1E-115, NT did not stimulate NTR gene activation but induced NTR mRNA destabilization after long term agonist exposure. In this cell line, NT binding dropped to 15% of control values and remained at this value after agonist treatment. The TH expression, which was originally activated upon NT agonist exposure, decreased to control values after prolonged agonist exposure.These observations combined with the data obtained from a complementary study with HT-29 cells (Souaze, F., Rostene, W., and Forgez, P. (1997) J. Biol. Chem. 272, 10087-10094) revealed the crucial role of agonist-induced receptorgene transcription in the maintenance of cell sensitivity. A model for G protein-coupled receptor regulation induced by prolong and intenseagonist stimulation is proposed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:42:46