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Titolo:
EXPRESSION AND NEUROPEPTIDERGIC CHARACTERIZATION OF ESTROGEN-RECEPTORS (ER-ALPHA AND ER-BETA) THROUGHOUT THE RAT-BRAIN - ANATOMICAL EVIDENCE OF DISTINCT ROLES OF EACH SUBTYPE
Autore:
LAFLAMME N; NAPPI RE; DROLET G; LABRIE C; RIVEST S;
Indirizzi:
CHU LAVAL,RES CTR,MOL ENDOCRINOL LAB,2705 BLVD LAURIER QUEBEC CITY PQG1V 4G2 CANADA CHU LAVAL,RES CTR,MOL ENDOCRINOL LAB QUEBEC CITY PQ G1V 4G2 CANADA UNIV LAVAL QUEBEC CITY PQ G1V 4G2 CANADA
Titolo Testata:
Journal of neurobiology
fascicolo: 3, volume: 36, anno: 1998,
pagine: 357 - 378
SICI:
0022-3034(1998)36:3<357:EANCOE>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICOTROPIN-RELEASING FACTOR; MESSENGER-RIBONUCLEIC-ACID; PARAVENTRICULAR NUCLEUS; INSITU HYBRIDIZATION; GENE-EXPRESSION; OXYTOCIN GENE; OVARIECTOMIZED RAT; INDUCED RESPONSES; RNA EXPRESSION; FEMALE RAT;
Keywords:
CORTICOTROPIN-RELEASING FACTOR; ENKEPHALIN; GASTRIN-RELATED PEPTIDE; HYPOTHALAMUS; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION HISTOCHEMISTRY; LUTEINIZING HORMONE-RELEASING HORMONE; OVULATORY CYCLE; OXYTOCIN; REPRODUCTION; VASOPRESSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
N. Laflamme et al., "EXPRESSION AND NEUROPEPTIDERGIC CHARACTERIZATION OF ESTROGEN-RECEPTORS (ER-ALPHA AND ER-BETA) THROUGHOUT THE RAT-BRAIN - ANATOMICAL EVIDENCE OF DISTINCT ROLES OF EACH SUBTYPE", Journal of neurobiology, 36(3), 1998, pp. 357-378

Abstract

The recent cloning of a second estrogen receptor (ER) provided a new tool to investigate and clarify how estrogens are capable of communicating with the brain and influence gene expression and neural function. The purpose of the present study was to define the neuroanatomical organization of each receptor subtype using a side-by-side approach and to characterize the cellular population(s) expressing the ER beta transcript in the endocrine hypothalamus using immunohistochemistry combined with in situ hybridization. Axonal transport inhibition was accomplished to cause neuropeptide accumulation into the cytoplasm and thus facilitate the detection of all positive luteinizing hormone-releasing hormone (LHRH), corticotropin-releasing factor (CRF), vasopressin (AVP), oxytocin (OT), gastrin-related peptide (GRP), and enkephalin (ENK) neurons. The genes encoding either ER alpha or -beta were expressed innumerous limbic-associated structures, and fine differences were found in terms of intensity and positive signal. Such phenomenon is best represented by the bed nucleus of the stria terminalis (BnST) and preoptic area/ anterior hypothalamus, where the expression pattern of both transcripts differed across subnuclei. The novel ER was also found to be expressed quite exclusively in other hypothalamic nuclei, includingthe supraoptic (SON) and selective compartments (magnocellular and autonomic divisions) of the paraventricular nucleus (PVN). A high percentage of the ER beta-expressing neurons located in the ventro- and dorsomedial PVN are of OT type; 40% of the OT-ir cells forming the medial magnocellular and ventromedial parvocellular PVN showed a clear hybridization signal for ER beta mRNA, whereas a lower percentage (15-20%) of OT neurons were positive in the caudal parvocellular PVN and no double-labeled cells were found in the rostral PVN and other regions of the brain with the exception of the SON. Very few AVP-ir neurons expressing ER beta transcript were found throughout the rat brain, although the medial PVN displayed some scattered double-labeled cells (<5%). Quite interestingly, the large majority of the ERP-positive cells in the caudal PVN were colocalized within CRF-ir perikarya. Indeed, more than60-80% of the CRF-containing cells located in the caudolateral division of the parvocellular PVN exhibited a positive hybridization signal for ER beta mRNA, whereas very few (<5%) neuroendocrine CRF-ir parvocellular neurons of the medial PVN expressed the gene encoding ER beta. A small percentage of ER beta-expressing cells in the dorsocaudal and ventromedial zones of the parvocellular PVN were also ENK positive. The ventral zone of the medial parvocellular PVN also displayed GRP-ir neurons, but no convincing hybridization signal for ER beta was detected in this neuronal population. Finally, as previously described for the gene encoding the classic ER, LHRH neurons of both intact and colchicine-pretreated animals did not express the novel estrogen receptor. This study shows a differential pattern of expression of both receptorsin the brain of intact rats and that ER beta is expressed at various levels in distinct neuropeptidergic populations, including OT, CRF, and ENK. The influence of estrogen in mediating genomic and neuronal responses may therefore take place within these specific cellular groups in the brains of cycling as well as intact male mammals. (C) 1998 JohnWiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 18:41:01