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Titolo:
DIFFERENTIAL-EFFECTS OF KINASE INHIBITOR AND ELECTRICAL STIMULUS ON ACTIVATION AND HISTONE H1 KINASE-ACTIVITY IN PIG OOCYTES
Autore:
LEAL CLV; LIU L;
Indirizzi:
LAB REPROD MED,LILLIE BLDG RM 321,7 MBL ST WOODS HOLE MA 02543 UNIV CONNECTICUT,DEPT ANIM SCI STORRS CT 06269 UNIV SAO PAULO,FAC VET MED,DEPT ANIM REPROD BR-05340000 SAO PAULO BRAZIL BABRAHAM INST,DEPT DEV & SIGNALING CAMBRIDGE CB2 4AT ENGLAND
Titolo Testata:
Animal reproduction science
fascicolo: 1, volume: 52, anno: 1998,
pagine: 51 - 61
SICI:
0378-4320(1998)52:1<51:DOKIAE>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
6-DIMETHYLAMINOPURINE 6-DMAP; CYCLIN SYNTHESIS; PORCINE OOCYTES; PROTEIN-KINASES; BOVINE OOCYTES; MOUSE OOCYTES; IN-VITRO; MATURATION; MPF; TRANSITION;
Keywords:
PARTHENOGENETIC ACTIVATION; PORCINE OOCYTE; 6-DMAP; HISTONE H1 KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
C.L.V. Leal e L. Liu, "DIFFERENTIAL-EFFECTS OF KINASE INHIBITOR AND ELECTRICAL STIMULUS ON ACTIVATION AND HISTONE H1 KINASE-ACTIVITY IN PIG OOCYTES", Animal reproduction science, 52(1), 1998, pp. 51-61

Abstract

The 6-DMAP (6-dimethylaminopurine) has been reported to accelerate and enhance the formation of pronuclei (activation) in non-aged MII mouse and bovine eggs. In this study, effects of chemical activation (CA) were evaluated using ethanol + 6-DMAP and electrical activation (EA) on pig oocytes matured for 36 h (newly matured) and 48 h. After CA, thefirst pronuclei developed within 2 h, with maximal pronuclear formation at 8 h (> 90%) for oocytes matured for either 36 or 48 h. In addition, chemically activated oocytes did not extrude the second polar body. After EA treatment, pronuclear formation began at 4-6 h and was significantly lower at 8 h in newly matured than aged oocytes (34% vs. 85%). Cleavage rare at 24 h after treatment was lower for oocytes treatedby CA (< 50%) than for EA (> 70%) and after 3 days of in vitro culture, fewer oocytes reached the four-cell stage in CA than in EA groups. No chemically activated oocytes developed beyond the four-cell stage; in contrast, EA resulted in development beyond this stage. Both CA andEA resulted in a drop of H1 kinase activity, but EA induced a more rapid reduction. With EA, H1 kinase activity rapidly declined but tendedto increase again at 8 h in oocytes matured for 36 h, which was different from all other groups in which the kinase activity remained low at 8 h post-activation. We conclude that different mechanisms are involved in pronuclear formation with CA and EA and that 6-DMAP-activated oocytes map not be suitable for the induction of normal parthenogeneticdevelopment in pigs. It is also suggested that the ability of pig oocytes to become fully activated may be related to the modification of H1 kinase activity during the aging process. These modifications to H1 kinase maybe necessary for parthenogenetic activation of in vitro matured oocytes and also for the completion of meiosis and cleavage. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:43:17