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Titolo:
DEFENSE GENE INDUCTION IN TOBACCO BY NITRIC-OXIDE, CYCLIC-GMP, AND CYCLIC ADP-RIBOSE
Autore:
DURNER J; WENDEHENNE D; KLESSIG DF;
Indirizzi:
RUTGERS STATE UNIV,WAKSMAN INST,190 FRELINGHUYSEN RD PISCATAWAY NJ 08854 RUTGERS STATE UNIV,WAKSMAN INST PISCATAWAY NJ 08854 RUTGERS STATE UNIV,DEPT MOL BIOL & BIOCHEM PISCATAWAY NJ 08854
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 17, volume: 95, anno: 1998,
pagine: 10328 - 10333
SICI:
0027-8424(1998)95:17<10328:DGIITB>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC ACQUIRED-RESISTANCE; PHENYLALANINE AMMONIA-LYASE; SALICYLIC-ACID; ACTIVE OXYGEN; DISEASE RESISTANCE; HYDROGEN-PEROXIDE; PLANTS; ARABIDOPSIS; EXPRESSION; RELEASE;
Keywords:
REACTIVE OXYGEN SPECIES; GUANYLATE CYCLASE; PATHOGENESIS-RELATED PROTEINS; CALCIUM; PLANT SIGNAL TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
J. Durner et al., "DEFENSE GENE INDUCTION IN TOBACCO BY NITRIC-OXIDE, CYCLIC-GMP, AND CYCLIC ADP-RIBOSE", Proceedings of the National Academy of Sciences of the United Statesof America, 95(17), 1998, pp. 10328-10333

Abstract

Reactive oxygen species are believed to perform multiple roles duringplant defense responses to microbial attack, acting in the initial defense and possibly as cellular signaling molecules. In animals, nitricoxide (NO) is an important redox-active signaling molecule. Here we show that infection of resistant, but not susceptible, tobacco with tobacco mosaic virus resulted in enhanced NO synthase (NOS) activity. Furthermore, administration of NO donors or recombinant mammalian NOS to tobacco plants or tobacco suspension cells triggered expression of thedefense-related genes encoding pathogenesis-related 1 protein and phenylalanine ammonia lyase (PAL). These genes were also induced by cyclic GMP (cGMP) and cyclic ADP-ribose, two molecules that can serve as second messengers for NO signaling in mammals. Consistent,vith cGMP acting as a second messenger in tobacco, NO treatment induced dramatic andtransient increases in endogenous cGMP levels. Furthermore, NO-induced activation of PAL was blocked by 6-anilino-5,8-quinolinedione and 1H-(1,2,4)-oxadiazole [4,3-a] quinoxalin-1-one, two inhibitors of guanylate cyclase. Although 6-anilino-5,8-quinolinedione fully blocked PAL activation, inhibition by 1H-(1,2,4)-oxadiazole [4,3-a] quinoxalin-1-one was not entirely complete, suggesting the existence of cGMP-independent, as well as cGMP-dependent, NO signaling. We conclude that severalcritical players of animal NO signaling are also operative in plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:45:16