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Titolo:
DIFFERENTIAL EXPRESSION OF ALTERNATIVE SPLICE VARIANTS OF BETA-ARRESTIN-1 AND BETA-ARRESTIN-2 IN RAT CENTRAL-NERVOUS-SYSTEM AND PERIPHERAL-TISSUES
Autore:
KOMORI N; CAIN SD; ROCH JM; MILLER KE; MATSUMOTO H;
Indirizzi:
UNIV OKLAHOMA,HLTH SCI CTR,DEPT BIOCHEM & MOL BIOL OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,HLTH SCI CTR,DEPT CELL BIOL OKLAHOMA CITY OK 73190
Titolo Testata:
European journal of neuroscience
fascicolo: 8, volume: 10, anno: 1998,
pagine: 2607 - 2616
SICI:
0953-816X(1998)10:8<2607:DEOASV>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
COUPLED RECEPTOR INTERNALIZATION; LIGHT-INDUCED PHOSPHORYLATION; BETA-ARRESTIN; S-ANTIGEN; BOVINE RETINA; WILD-TYPE; PROTEIN; RHODOPSIN; BINDING; FAMILY;
Keywords:
G-PROTEIN-COUPLED RECEPTOR (GPCR); GENOMIC DNA SEQUENCE; ISOELECTRIC HETEROGENEITY; RT-PCR; CDNA SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
N. Komori et al., "DIFFERENTIAL EXPRESSION OF ALTERNATIVE SPLICE VARIANTS OF BETA-ARRESTIN-1 AND BETA-ARRESTIN-2 IN RAT CENTRAL-NERVOUS-SYSTEM AND PERIPHERAL-TISSUES", European journal of neuroscience, 10(8), 1998, pp. 2607-2616

Abstract

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors, including rhodopsin and beta(2)-adrenergic receptor. Unlike in human and cow, splice variants of this protein family in rat have not been studied extensively, and there has been no report on their existence at protein level. Hence, a previous report by others on the localization of both beta-arrestin-1 and -2 in a wide range of innervated rat tissues could imply their broad receptor specificity. In this report we show the presence of two alternatively spliced forms of beta-arrestin-1 in several rat tissues using both reverse transcription-polymerasechain reaction and Western immunoblot. Splicing of beta-arrestin-1 pre-mRNA appears to be subject to differential regulation between the rat CNS and peripheral tissues. In contrast, we detected no splice variants of beta-arrestin-2 in rat. A comparison of the genomic DNA sequences of bovine and rat beta-arrestin-2, where the splicing of bovine beta-arrestin-2 mRNA has been reported, revealed a high degree of homology in their organization of exons and introns as well as certain differences that might be responsible for the different processing of beta-arrestin-2 mRNA in the two species. Our two-dimensional isoelectric focusing gels using rat spinal cord and heart tissues demonstrate isoelectric heterogeneity of rat beta-arrestin-1, suggesting that beta-arrestin-1 is subject to post-translational modification unlike beta-arrestin-2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 16:53:34