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Titolo:
INTRACELLULAR CALCIUM RESPONSES IN BOVINE OOCYTES INDUCED BY SPERMATOZOA AND BY REAGENTS
Autore:
NAKADA K; MIZUNO J;
Indirizzi:
RAKUNO GAKUEN UNIV,DEPT VET OBSTET & GYNECOL EBETSU HOKKAIDO 069850 JAPAN MIC CO LTD,RES LAB NASU TOCHIGI 3293224 JAPAN
Titolo Testata:
Theriogenology
fascicolo: 2, volume: 50, anno: 1998,
pagine: 269 - 282
SICI:
0093-691X(1998)50:2<269:ICRIBO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
GOLDEN-HAMSTER EGGS; INOSITOL TRISPHOSPHATE; PARTHENOGENETIC DEVELOPMENT; CA2+ OSCILLATIONS; MOUSE OOCYTES; RABBIT EGGS; RELEASE; ACTIVATION; FERTILIZATION; TRANSIENTS;
Keywords:
BOVINE OOCYTES; CALCIUM; FERTILIZATION; PARTHENOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
K. Nakada e J. Mizuno, "INTRACELLULAR CALCIUM RESPONSES IN BOVINE OOCYTES INDUCED BY SPERMATOZOA AND BY REAGENTS", Theriogenology, 50(2), 1998, pp. 269-282

Abstract

The objectives of the present study were to clarify and compare the characteristics of the transient rises in intracellular calcium concentrations ([Ca-i(2+])) induced either by spermatozoa or by stimulation with artificial activators in bovine oocytes. These transient rises in [Ca2+](i) in oocytes matured in vitro were recorded with Ca2+ imaging using the Ca2+ indicator fura-2. During fertilization, a series of transient rises in [Ca2+](i) was observed. The first Ca2+ response peakedat a concentration of 521+/- nM (n=20) and lasted for 4 min, while the subsequent Ca2+ responses were significantly smaller and shorter, with a peak of 368+/13 nM (n=23) and a duration of 2 min. Injection of inositol 1,4,5- triphosphate (InsP(3)) into unfertilized oocytes causeda transient rise in [Ca2+]i ina dose-dependent manner. The maximum response was induced by 20 nA x 1 sec injection of InsP3. Thimerosal, a sulfhydryl reagent, induced the repetitive transient rises in [Ca2+](i). The peak and the duration of the rises in [Ca2+](i) induced by InsP(3) or thimerosal were smaller and shorter, respectively, than those of the first rise induced by spermatozoa. Ethanol and Ca2+ ionophore IA23187, which are general parthenogenetic activators of unfertilized oocytes, each induced a single transient rise in [Ca2+](i). The durationof the rise in [Ca2+](i) by ethanol or Ca2+ ionophore was significantly longer than that by spermatozoa at fertilization, although the peaks were smaller. These results clarified the characteristics of the rises in [Ca2+](i) induced by spermatozoa and by several artificial reagents, and showed that the first rise in [Ca2+](i) induced by spermatozoa had a higher peak [Ca2+](i) and a longer duration compared with eachthe subsequent rises in [Ca2+](i) and the rises in [Ca2+](i) induced by artificial reagents, These indicate that a mode like as the first rise in [Ca2+]i induced by spermatozoa is an effective trigger for artificial activation of oocytes. (C) 1998 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 05:25:58