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Titolo:
LOCALIZATION OF A PROTEIN CORE-SPECIFIC EPITOPE FROM GASTROINTESTINALMUCIN (MUC2) - THE EFFECT OF EPITOPE IMMOBILIZATION ON ANTIBODY RECOGNITION
Autore:
URAY K; PRICE MR; HUDECZ F;
Indirizzi:
HUNGARIAN ACAD SCI,RES GRP PEPTIDE CHEM,POB 32 H-1518 BUDAPEST 112 HUNGARY HUNGARIAN ACAD SCI,RES GRP PEPTIDE CHEM H-1518 BUDAPEST 112 HUNGARY UNIV NOTTINGHAM,DEPT PHARMACEUT SCI,CANC RES LAB NOTTINGHAM NG7 2RD ENGLAND
Titolo Testata:
Journal of peptide science
fascicolo: 5, volume: 4, anno: 1998,
pagine: 319 - 326
SICI:
1075-2617(1998)4:5<319:LOAPCE>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE SYNTHESIS; MOLECULAR-CLONING; PEPTIDE-SYNTHESIS; SEQUENCE; CDNAS; GENE; ACID;
Keywords:
MUC2 MUCIN; SYNTHETIC PEPTIDES; ANTIBODY RECOGNITION IN SOLUTION; ANTIBODY RECOGNITION ON IMMOBILIZED PEPTIDES; B CELL EPITOPE OF MUC2 MUCIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
K. Uray et al., "LOCALIZATION OF A PROTEIN CORE-SPECIFIC EPITOPE FROM GASTROINTESTINALMUCIN (MUC2) - THE EFFECT OF EPITOPE IMMOBILIZATION ON ANTIBODY RECOGNITION", Journal of peptide science, 4(5), 1998, pp. 319-326

Abstract

Human intestinal mucins are high molecular weight glycoproteins whichprotect and lubricate the epithelium of the gastrointestinal tract. In cases of malignant disease, mucins are abnormally expressed, overproduced or underglycosylated. This feature may enable the mucins to serve as tumour markers. The MUC2 mucin largely consists of a variable number of tandem repeats of a 23 amino acid sequence, (1)PTTTPITTTTTVTPTPTPTGTQT(23). In this study we have localised the minimal and the optimal epitope within this region by the previously developed protein corespecific 996 monoclonal antibody using synthetic peptides. Several overlapping and truncated peptides related to the tandem repeat unit have been prepared by solid-phase methodology. Other mucin peptides were synthesised on the tips of polyethylene pins, and these remained C-terminally attached to the pins for comparative investigations. The interaction of the 996 monoclonal antibody with the synthetic peptides was studied either in solution by competition RIA or on immobilised peptides by indirect ELISA experiments. These experiments show that the minimal epitope recognised by the 996 antibody is the Ac-(19)TGTQ(22) (IC50=3100 mu M in solution). For the optimal 996 antibody binding in solution the (16)PTPTGTQ(22) heptapeptide (IC50=3 mu M) is required. (C) 1998 European Peptide Society and John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:25:33