Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE PATHOPHYSIOLOGICAL SIGNIFICANCE OF NONDESMOGLEIN TARGETS OF PEMPHIGUS AUTOIMMUNITY - DEVELOPMENT OF ANTIBODIES AGAINST KERATINOCYTE CHOLINERGIC RECEPTORS IN PATIENTS WITH PEMPHIGUS-VULGARIS AND PEMPHIGUS FOLIACEUS
Autore:
NGUYEN VT; LEE TX; NDOYE A; SHULTZ LD; PITTELKOW MR; DAHL MV; LYNCH PJ; GRANDO SA;
Indirizzi:
UNIV CALIF DAVIS,MED CTR,DEPT DERMATOL,4860 Y ST,SUITE 3400 SACRAMENTO CA 95817 UNIV CALIF DAVIS,MED CTR,DEPT DERMATOL SACRAMENTO CA 95817 UNIV MINNESOTA,DEPT DERMATOL MINNEAPOLIS MN 55455 JACKSON LAB BAR HARBOR ME 04609 MAYO CLIN,DEPT DERMATOL MINNEAPOLIS MN 00000
Titolo Testata:
Archives of dermatology
fascicolo: 8, volume: 134, anno: 1998,
pagine: 971 - 980
SICI:
0003-987X(1998)134:8<971:TPSONT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCARINIC ACETYLCHOLINE-RECEPTORS; CELL CARCINOMA LINE; PROTEIN-KINASE-C; MYASTHENIA-GRAVIS; INOSITOL 1,4,5-TRISPHOSPHATE; INTRACELLULAR CALCIUM; DESMOSOMAL PLAQUE; DJM-1 CELLS; ADHESION; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
72
Recensione:
Indirizzi per estratti:
Citazione:
V.T. Nguyen et al., "THE PATHOPHYSIOLOGICAL SIGNIFICANCE OF NONDESMOGLEIN TARGETS OF PEMPHIGUS AUTOIMMUNITY - DEVELOPMENT OF ANTIBODIES AGAINST KERATINOCYTE CHOLINERGIC RECEPTORS IN PATIENTS WITH PEMPHIGUS-VULGARIS AND PEMPHIGUS FOLIACEUS", Archives of dermatology, 134(8), 1998, pp. 971-980

Abstract

Objectives: To determine whether nondesmoglein (non-Dsg) autoantibodies are pathogenic and whether they recognize keratinocyte cholinergic receptors that control cell adhesion because antikeratinocyte autoimmunity in patients with pemphigus vulgaris is not limited to the development of autoantibodies to Dsg. Design: To determine whether non-Dsg autoantibodies are pathogenic, we sought to induce pemphigus in genetically engineered neonatal mice lacking Dsg using pemphigus vulgaris IgGsthat did not cross-react with Dsg 1. To determine whether pemphigus autoimmunity involves keratinocyte cholinergic receptors, the latter were separated from cell membranes of human keratinocytes, tagged with the covalent label [H-3]propylbenzilylcholine mustard, and used as an antigen in a radioimmunoprecipitation assay of 34 pemphigus vulgaris and 6 pemphigus foliaceus serum samples. Setting: The dermatologic clinics of the University of Minnesota, Minneapolis; the Mayo Clinic, Rochester, Minn; and the University of California-Davis Medical Center, Sacramento. Patients: Serum samples were collected from 34 patients with pemphigus vulgaris and 6 patients with pemphigus foliaceus (aged 31-89years) and from 7 age-similar patients of both sexes with nonpemphisus blistering or the following immune-mediated conditions: pemphigoid gestationis, bullous drug eruption, lupus erythematosus, erythema nodosum, urticaria, acute contact dermatitis, and skin ulcers. Main OutcomeMeasures: Clinical, laboratory, and histopathologic findings. Results: Extensive skin blistering accompanied by the Nikolsky sign and suprabasilar acantholysis was induced in the Dsg3(nul)l mice that received pemphigus, but not normal human IgGs. In the radioimmunoprecipitation assays for reactivity with cholinergic receptors, the mean radioactivity precipitated by pemphigus serum samples significantly exceeded bothnormal- and disease-control levels (P = .001-.02). The mean individual levels of radioactivity precipitated by 34 pemphigus vulgaris and pemphigus foliaceus serum samples (85%) exceeded control values by a mean of approximately 2.6 times. Conclusions: Autoantibodies to keratinocyte cell-surface molecules other than Dsg 1 and Dsg 3 can induce clinical features of pemphigus vulgaris. Patients with pemphigus vulgaris and those with pemphigus foliaceus develop IgG antibodies that precipitate radiolabeled cholinergic receptors. Because these receptors control keratinocyte adhesion and motility, their inactivation by autoantibodies may elicit intracellular signals that cause disassembly of desmosomes, leading to acantholysis and blistering.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 06:55:15