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Titolo:
ANTIOXIDANT ENZYMES IN NICOTIANA CELLS CONTAINING AN IPOMOEA PEROXIDASE GENE
Autore:
YUN BW; HUH GH; KWON SY; LEE HS; JO JK; KWAK SS;
Indirizzi:
KRIBB,PLANT BIOCHEM RES UNIT,POB 115 TAEJON 305600 SOUTH KOREA KRIBB,PLANT BIOCHEM RES UNIT TAEJON 305600 SOUTH KOREA KYUNGPOOK NATL UNIV,DEPT ANIM SCI TAEGU 702701 SOUTH KOREA
Titolo Testata:
Phytochemistry
fascicolo: 8, volume: 48, anno: 1998,
pagine: 1287 - 1290
SICI:
0031-9422(1998)48:8<1287:AEINCC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUSPENSION-CULTURES; MOLECULAR-CLONING; SWEET-POTATO; EXPRESSION;
Keywords:
IPOMOEA BATATAS; CONVOLVULACEAE; NICOTIANA TABACUM; SOLANACEAE; TRANSGENIC PLANTS; TRANSGENIC CELL LINES; PEROXIDASE; SUPEROXIDE DISMUTASE; CATALASE; GLUTATHIONE REDUCTASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
B.W. Yun et al., "ANTIOXIDANT ENZYMES IN NICOTIANA CELLS CONTAINING AN IPOMOEA PEROXIDASE GENE", Phytochemistry, 48(8), 1998, pp. 1287-1290

Abstract

The levels of the antioxidant enzymes, peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR), were investigated in seven cell lines (referred to as transgenic tobacco celllines) derived from transgenic tobacco (Nicotiana tabacum) plants carrying a sweet potato (Ipomoea batatas) anionic POD cDNA (swpal). Transgenic tobacco cell lines were induced from the leaf tissues of transgenic plants on MS medium supplemented with 1 mg 1(-1) 2,4-D. A novel isoenzyme encoded by the swpal cDNA was detected on the native-PAGE in all transgenic tobacco cell lines. Average POD activity in transgenic cell lines was ca 1.3 times higher than that of control cell lines, whereas the other three antioxidant enzymes showed a slightly lower levelin transgenic cell lines. The POD specific activity in each transgenic cell line had a high correlation with that of leaves in the originaltransgenic plants (r = 0.63). The ratio of in vitro cell lines to plant leaves in specific activities of four antioxidant enzymes showed a considerable difference: 2.5 in POD activity, 2.1 in SOD activity, 0.05 in CAT activity and 0.9 in GR activity. These results suggest that the antioxidative mechanism between in vitro cultured cells and intact plants may be differently regulated. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 17:20:46