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Titolo:
ENDOTOXIN-STIMULATED ALVEOLAR MACROPHAGE RECRUITMENT OF NEUTROPHILS AND MODULATION WITH EXOGENOUS SURFACTANT
Autore:
FINCK CM; HODELL MG; MARX WH; PASKANIK AM; MCGRAW DJ; LUTZ CJ; GATTO LA; PICONE AL; NIEMAN GF;
Indirizzi:
SUNY HLTH SCI CTR,DEPT SURG,750 E ADAMS ST SYRACUSE NY 13210 SUNY HLTH SCI CTR,DEPT SURG SYRACUSE NY 13210
Titolo Testata:
Critical care medicine
fascicolo: 8, volume: 26, anno: 1998,
pagine: 1414 - 1418
SICI:
0090-3493(1998)26:8<1414:EAMRON>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY-DISTRESS-SYNDROME; GRAM-NEGATIVE SEPSIS; SYNTHETIC SURFACTANT; ACCUMULATION; MIGRATION; CYTOKINES; EXOSURF; ARDS; LUNG;
Keywords:
ALVEOLAR MACROPHAGE; NEUTROPHILS; LEUKOCYTE; CHEMOTAXIS; SURFACTANT; ACUTE RESPIRATORY DISTRESS SYNDROME; ENDOTOXIN; CYTOKINE; LUNG PATHOLOGY; ACUTE LUNG INJURY; CHEMOKINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
C.M. Finck et al., "ENDOTOXIN-STIMULATED ALVEOLAR MACROPHAGE RECRUITMENT OF NEUTROPHILS AND MODULATION WITH EXOGENOUS SURFACTANT", Critical care medicine, 26(8), 1998, pp. 1414-1418

Abstract

Objective: To determine whether endotoxin-stimulated alveolar macrophages would attract neutrophils and whether exogenous surfactant treatment would modulate this chemoattraction. Design: Alveolar macrophages were harvested from bronchoalveolar lavage fluid and neutrophils from the blood of anesthetized guinea pigs. Subjects: Hartley guinea pigs. Interventions: Alveolar macrophages were suspended in RPMI 1640 and stimulated with 1 mu g/mL of lipopolysaccharide (LPS), the supernatant removed and the alveolar macrophages were incubated in either RPMI or RPMI with surfactant at two different doses (292 mu g/mL or 875 mu g/mL) for 16 hrs. Measurements and Main Results: The supernatant was extracted from the alveolar macrophages and placed in a chemotaxis plate and the migration of neutrophils was measured. Chemotaxis of all cell types to be tested was measured by a change of absorbance on a microplate reader set at 492 nm. Results were compared with alveolar macrophages not stimulated with LPS, RPMI alone, and N formyl-methionyl-leucyl-phenylalanine (FMLP). The supernatant of the stimulated alveolar macrophages increased neutrophil chemotaxis as compared with unstimulated alveolar macrophages, and RPMI (p < .05). surfactant treatment with 292 mu g/mL significantly decreased LPS-stimulated alveolar macrophages induced neutrophil chemotaxis. Treatment with 875 mu g/mL of surfactant did not alter neutrophil chemotaxis. Conclusions: Alveolar macrophagesstimulation with LPS increased the chemotaxis of neutrophils. Treatment with surfactant at a concentration of 875 mu g/mL did not alter neutrophil migration; however, treatment with 292 mu g/mL significantly decreased neutrophil chemotaxis suggesting that at low concentrations, surfactant inhibits chemokine release and may reduce pulmonary neutrophil sequestration in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:00:24