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Titolo:
BIOCHEMICAL AND ULTRA-STRUCTURAL REACTIONS TO PARENTERAL-NUTRITION WITH 2 DIFFERENT FAT EMULSIONS IN RATS
Autore:
ROTH B; EKELUND M; FAN BG; HAGERSTRAND I; SALEHI A; LUNDQUIST I; NILSSONEHLE P;
Indirizzi:
UNIV LUND HOSP,DEPT ANESTHESIOL S-22185 LUND SWEDEN UNIV LUND HOSP,DEPT SURG S-22185 LUND SWEDEN UNIV LUND HOSP,DEPT CLIN PATHOL S-22185 LUND SWEDEN UNIV LUND HOSP,DEPT PHARMACOL S-22185 LUND SWEDEN UNIV LUND HOSP,DEPT CLIN CHEM S-22185 LUND SWEDEN
Titolo Testata:
Intensive care medicine
fascicolo: 7, volume: 24, anno: 1998,
pagine: 716 - 724
SICI:
0342-4642(1998)24:7<716:BAURTP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPOPROTEIN-LIPASE; HEPATIC LIPASE; MEDIUM-CHAIN; RETICULOENDOTHELIAL SYSTEM; LIPID EMULSIONS; TRIGLYCERIDE; METABOLISM; DEPOSITION; INFUSION; HEALTHY;
Keywords:
INTRAVENOUS FAT EMULSIONS; KUPFFER CELLS; HISTOLOGY; FATTY LIVER; LIPOPROTEIN LIPASE; LYSOSOMAL ENZYMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
B. Roth et al., "BIOCHEMICAL AND ULTRA-STRUCTURAL REACTIONS TO PARENTERAL-NUTRITION WITH 2 DIFFERENT FAT EMULSIONS IN RATS", Intensive care medicine, 24(7), 1998, pp. 716-724

Abstract

Objective: To compare the effects on fat metabolism and Kupffer cell morphology by total parenteral nutrition (TPN) with two different fat emulsions. Design: Thirty-two male Sprague-Dawley rats, divided into three groups, were investigated. Rats fed orally were used as a reference group. and a group of rats receiving TPN with fat emulsions containing pure long-chain triglycerides (LCT) was compared to a group of rats receiving fat emulsions containing both long-chain triglycerides andmedium-chain triglycerides (MCT/LCT), The TPN regimens were equicaloric and administered continuously via a jugular catheter for 10 days. Interventions: After suffocation, blood of the rats was collected for the determination of serum lipids. Epididymal fat and heart were collected for the analysis of lipoprotein lipase (LPL) activities, and liverspecimens were saved for analyses of hepatic triglyceride concentration, as well as activities of hepatic lipase (HL) and lysosomal enzymes. Light and electron microscopy were used for examination of the Kupffer cell reaction. Results: Directly after termination of parenteral feeding, the levels of serum triglycerides and high density lipoprotein (HDL) triglycerides were higher in the MCT/LCT group than in the LCT group, while no differences concerning cholesterol and phospholipid concentrations were found. No significant difference in liver steatosis was found between the two TPN groups. Comparison of the TPN groups showed that the MCT/LCT group had significantly decreased LPL activity in adipose tissue, while the LCT group had significantly increased LPL activity in the heart. The activity of HL was low in both groups, but significantly lower in the LCT group. Lipid accumulation and an increased number of lysosomes were found in all Kupffer cell when TPN with LCTemulsions was used. Moreover, TPN induced a pronounced increase in lysosomal enzyme activities, but there was no notable difference betweenLCT and MCT/LCT effects. Conclusions: Compared to treatment with pureLCT emulsions treatment with MCT/LCT emulsions evoked weaker biochemical reactions in terms of lower activity of Lipoprotein lipase in fat and heart together with higher serum and HDL triglyceride levels. Morphological signs of increased Kupffer cell activity such as the appearance of multiple lysosomes and fat vacuoles in the cytoplasm followed treatment with pure LCT emulsions. However, both TPN groups showed a marked increase in activities of liver lysosomal enzymes.

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Documento generato il 26/09/20 alle ore 23:52:40